Determination of Gallic Acid in Rat Plasma by LC-MS-MS

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• 作者：Rui Song ; Lei Xu ; Zunjian Zhang ; Yuan Tian ; Fengguo Xu and Haijuan Dong
• 关键词：Column liquid chromatography ; Tandem mass spectrometry ; Gallic acid ; Rhubarb ; Pharmacokinetics
• 刊名：Chromatographia
• 出版年：2010
• 出版时间：June, 2010
• 年：2010
• 卷：71
• 期：11-12
• 页码：1107-1111
• 全文大小：320.0 KB

文摘

Liquid chromatography–electrospray ionization tandem mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of gallic acid in rat plasma. Sample pretreatment involved a one-step extraction using ethyl acetate with protocatechic acid as internal standard. Separation was on a C₁₈ column using an isocratic mobile phase, consisting of methanol-0.1 % aqueous formic acid (40:60, v/v) at 0.2 mL min−1. The stability of gallic acid was evaluated in acidified and non-acidified plasma. The method was validated then successfully applied to a pharmacokinetic study in rats after oral administration of rhubarb extract.