Selenium-enriched exopolysaccharides improve skeletal muscle glucose uptake of diabetic KKAy mice via AMPK pathway

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• 作者：Xihong Zhou (1)
  Jingqing Chen (1)
  Fengqin Wang (1)
  Hangxian Yang (1)
  Ren Yang (1)
  Xinxia Wang (1)
  Yizhen Wang (1)
  
• 关键词：EPS ; Diabetes ; Skeletal muscle ; AMPKα2 ; PGC ; 1α
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：70
• 期：2
• 页码：547-554
• 全文大小：
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• 作者单位：Xihong Zhou (1)
  Jingqing Chen (1)
  Fengqin Wang (1)
  Hangxian Yang (1)
  Ren Yang (1)
  Xinxia Wang (1)
  Yizhen Wang (1)
  
  1. Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Institute of Feed Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People Republic of China
  
• ISSN：1877-8755

文摘

Selenium-enriched exopolysaccharides (EPS) produced by Enterobacter cloacae Z0206 have been proven to possess effect on reducing blood glucose level in diabetic mice. To investigate the specific mechanism, we studied the effects of oral supply with EPS on skeletal muscle glucose transportation and consumption in high-fat-diet-induced diabetic KKAy mice. We found that EPS supplementation increased expressions of glucose transporter 4 (Glut4), hexokinase 2 (hk2), phosphorylation of AMP-activated kinase subunit α2 (pAMPKα2), and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and increased expression of characteristic protein of oxidative fibers such as troponin I and cytochrome c (Cytc). Furthermore, we found that EPS increased glucose uptake and expressions of pAMPKα2 and PGC-1α in palmitic acid (PA)-induced C2C12 cells. However, while EPS inhibited AMPKα2 with interference RNA (iRNA), effects of EPS on the improvement of glucose uptake diminished. These results indicated that EPS may improve skeletal muscle glucose uptake of diabetic KKAy mice through AMPKα2-PGC-1α pathway.