Enhanced production of tetramethylpyrazine in Bacillus licheniformis BL1 by bdhA disruption and 2,3-butanediol supplementation

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• 作者：Wu Meng ; Dongguang Xiao ; Ruiming Wang
• 关键词：Bacillus licheniformis ; bdhA gene ; Metabolic engineering ; TMP ; Yield
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：32
• 期：3
• 全文大小：699 KB
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• 作者单位：Wu Meng (1) (2)
  Dongguang Xiao (2)
  Ruiming Wang (1)
  
  1. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology, Jinan, 250353, Shandong, China
  2. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

The 2,3-butanediol (2,3-BD) dehydrogenase gene (bdhA) of Bacillus licheniformis BL1 was disrupted to construct the tetramethylpyrazine (TMP)-producing BLA strain. During microaerobic fermentation, the bdhA-disrupted BLA strain produced 46.98 g TMP/l, and this yield was 23.99 % higher than that produced by the parent BL1 strain. In addition, the yield of acetoin, which is a TMP precursor, also increased by 28.98 % in BLA. The TMP production by BL1 was enhanced by supplementing the fermentation medium with 2,3-BD. The yield of TMP improved from 37.89 to 44.77 g/l as the concentration of 2,3-BD increased from 0 to 2 g/l. The maximum TMP and acetoin yields increased by 18.16 and 17.87 %, respectively with the increase in 2,3-BD concentration from 0 to 2 g/l. However, no increase was observed when the concentration of 2,3-BD in the matrix was ≥3 g/l. This study provides a valuable strategy to enhance TMP and acetoin productivity of mutagenic strains by gene manipulation and optimizing fermentation conditions. Keywords Bacillus licheniformis bdhA gene Metabolic engineering TMP Yield