Lactic acid production from hemicellulosic hydrolyzate by cells of Lactobacillus bifermentans immobilized in Ca-alginate using response surface methodology

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• 作者：Sébastien Givry ; Vincent Prevot and Francis Duchiron
• 关键词：Ca ; alginate ; Immobilization ; MRS medium ; Lactic acid ; Lactobacillus bifermentans ; Optimization ; Production
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2008
• 出版时间：June, 2008
• 年：2008
• 卷：24
• 期：6
• 页码：745-752
• 全文大小：432.2 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Consumption of hexoses/pentoses and production of lactic acid by Lactobacillus bifermentans were investigated in optimized culture medium and hemicellulosic hydrolyzates. The hydrolyzate used had the following composition (expressed in gL?1): xylose 50 ± 5 gL?1; glucose 18 ± 3 gL?1; arabinose 29 ± 5 gL?1. The immobilization experiments were conducted with microbial cells entrapped in calcium alginate beads. The results indicate that maximum concentrations of lactic acid were produced after 54 h of fermentation. All glucose and arabinose in wheat bran hydrolyzate were consumed during fermentation. Only xylose was not completely consumed. The substrate consumption rate was 3.2 gh?1, 1.9 gh?1, 1.6 gh?1 respectively for glucose, arabinose, and xylose. The optimized culture condition gave a lactic acid concentration and metabolic yield of 62.77 gL?1 and 0.83 gg?1. These parameters improved to 41.3 gL?1 and 0.47 gg?1 respectively, when cell free was used.