The rumen microbial metaproteome as revealed by SDS-PAGE

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• 作者：Timothy J. Snelling ; R. John Wallace
• 关键词：Cattle ; Proteomics ; Rumen ; Sheep
• 刊名：BMC Microbiology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：1698KB
• 刊物主题：Microbiology; Biological Microscopy; Mycology; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180
• 卷排序：17

文摘

BackgroundRuminal digestion is carried out by large numbers of bacteria, archaea, protozoa and fungi. Understanding the microbiota is important because ruminal fermentation dictates the efficiency of feed utilisation by the animal and is also responsible for major emissions of the greenhouse gas, methane. Recent metagenomic and metatranscriptomic studies have helped to elucidate many features of the composition and activity of the microbiota. The metaproteome provides complementary information to these other –omics technologies. The aim of this study was to explore the metaproteome of bovine and ovine ruminal digesta using 2D SDS-PAGE.