Probiotic attributes of Lactobacillus fermentum isolated from human feces and dairy products

详细信息    查看全文

• 作者：Ann Catherine Archer ; Prakash M. Halami
• 关键词：Probiotics ; L. fermentum ; Molecular fingerprinting ; Probiotic marker genes
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：99
• 期：19
• 页码：8113-8123
• 全文大小：257 KB
• 参考文献：Badarinath V, Halami PM (2009) Evaluation of bacteriocinogenic lactic acid bacteria isolated from fermented milk and idli batter for probiotic applications. Int J Prob Preb 4:33-0
  Bao Y, Zhang Y, Zhang Y, Liu Y, Wang S, Dong X, Zhang H (2010) Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control 21:695-01. doi:10.-016/?j.?foodcont.-009.-0.-10 CrossRef
  Begley M, Gahan CG, Hill C (2005) The interaction between bacteria and bile. FEMS Microbiol Rev 29:625-51. doi:10.-016/?j.?femsre.-004.-9.-03 CrossRef PubMed
  Begley M, Hill C, Gahan CG (2006) Bile salt hydrolase activity in probiotics. Appl Environ Microbiol 72:1729-738. doi:10.-128/?AEM.-2.-.-729-1738.-006 PubMed Central CrossRef PubMed
  Bover-Cid S, Holzapfel WH (1999) Improved screening procedure for biogenic amine production by lactic acid bacteria. Int J Food Microbiol 53:33-1. doi:10.-016/?S0168-1605(00)00526-2 CrossRef PubMed
  Chagnaud P, Machinis K, Coutte AL, Marecat A, Mercenier A (2001) Rapid PCR-based procedure to identify lactic acid bacteria: application to six common Lactobacillus species. J Microbiol Methods 44:139-48. doi:10.-016/?S0167-7012(00)00244-X CrossRef PubMed
  Cotter PD, Hill C (2003) Surviving the acid test: responses of Gram-positive bacteria to low pH. Microbiol Mol Biol Rev 67:429-53. doi:10.-128/?MMBR.-7.-.-29-453.-003 PubMed Central CrossRef PubMed
  Devi SM, Halami PM (2011) Detection and characterization of pediocin PA-1/AcH like bacteriocin producing lactic acid bacteria. Curr Microbiol 62:181-85. doi:10.-007/?s00284-011-9963-8 CrossRef
  Dickson EM, Riggio MP, Macpherson L (2005) A novel species-specific PCR assay for identifying Lactobacillus fermentum. J Med Microbiol 54:299-03. doi:10.-099/?jmm.-.-5770-0 CrossRef PubMed
  Draksler D, Gonzáles S, Oliver G (2004) Preliminary assays for the development of a probiotic for goats. Reprod Nutr Dev 44:397-05. doi:10.-051/?rnd:-004046 CrossRef PubMed
  Du Toit M, Franz CMAP, Dicks LMT, Schillinger U, Haberer P, Warlies B, Holzapfel WH (1998) Characterisation and selection of probiotic lactobacilli for a preliminary minipig feeding trial and their effect on serum cholesterol levels, faeces pH and faeces moisture content. Int J Food Microbiol 40:93-04. doi:10.-016/?S0168-1605(98)00024-5 CrossRef PubMed
  Ferrero M, Cesena C, Morelli L, Scolari G, Vescovo M (1996) Molecular characterization of Lactobacillus casei strains. FEMS Microbiol Lett 140:215-19. doi:10.-111/?j.-574-6968.-996.?tb08339.?x CrossRef
  Gevers D, Huys G, Swings J (2001) Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31-6. doi:10.-111/?j.-574-6968.-001.?tb10921.?x CrossRef PubMed
  Guarner F, Malagelada J-R (2003) Gut flora in health and disease. The?Lancet 361:512-19. doi:10.-016/?S0140-6736(03)12489-0
  Harzallah D, Belhadj H (2013) Lactic acid bacteria as probiotics: characteristics, selection criteria and role in immunomodulation of human GI mucosal barrier. Lactic Acid Bacteria–R & D for Food, Health Livest Purp 197-217
  Hemarajata P, Versalovic J (2012) Effects of probiotics on gut microbiota: mechanisms of intestinal immunomodulation and neuromodulation. Ther Adv Gastroenterol. doi:10.-177/-756283X12459294-/span>
  Jiménez E, Langa S, Martín V, Arroyo R, Martín R, Fernández L, Rodríguez JM (2010) Complete genome sequence of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk. J Bacteriol 192:4800-800. doi:10.-128/?JB.-0702-10
  Joint FAO (2002) WHO working group report on drafting guidelines for the evaluation of probiotics in food. London, Ontario, Canada, 30
  Joosten HMLJ, Northolt MD (1989) Detection, growth, and amine-producing capacity of lactobacilli in cheese. Appl Environ Microbiol 55:2356-359PubMed Central PubMed
  Kaushik JK, Kumar A, Duary RK, Mohanty AK, Grover S, Batish VK (2009) Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PloS One 4:1-1. doi:10.-371/?journal.?pone.-008099 CrossRef
  Lebeer S, Vanderleyden J, De Keersmaecker SC (2008) Genes and molecules of lactobacilli supporting probiotic action. Microbiol Mol Biol Rev 72:728-64. doi:10.-128/?MMBR.-0017-08 PubMed Central CrossRef PubMed
  Li S, Zhao Y, Zhang L, Zhang X, Huang L, Li D, Wang Q (2012) Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Food Chem 135:1914-919. doi:10.-016/?j.?foodchem.-012.-6.-48 CrossRef PubMed
  Ma?é J, Lorén V, Pedrosa E, Ojanguren I, Xaus J, Cabré E, Gassull MA (2009) Lactobacillus fermentum CECT 5716 prevents and reverts intestinal damage on TNBS‐induced colitis in mice. Inflamm Bowel Dis 15:1155-163. doi:10.-002/?ibd.-0908 CrossRef PubMed
  Mathara JM, Schillinger U, Guigas C, Franz C, Kutima PM, Mbugua SK, Sin HK, Holzapfel WH (200
• 作者单位：Ann Catherine Archer (1)
  Prakash M. Halami (1)
  
  1. Microbiology and Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysore, 570020, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The objective of this study was to characterize native Lactobacillus fermentum isolates for their probiotic attributes. Accordingly, 12 L. fermentum isolates selected from indigenous fermented dairy products and infant fecal samples were evaluated for their probiotic properties by in vitro and PCR methods. The cultures exhibited high tolerance to acid and bile as well as survival in simulated transit fluids (above 70 %). Cell surface hydrophobicity was in the range of 0.55-7.69 % for xylene and 0.45-7.12 % for hexadecane, whereas auto-aggregation ranged between 9 and 62 %. Isolates exhibited efficient binding to mucin and fibronectin, bile salt hydrolase activity, cholesterol assimilation (49-6 %), and radical scavenging activity (37-7 %). The isolates demonstrated antibacterial activity against Listeria monocytogenes Scott A and Micrococcus luteus ATCC 9341. Molecular fingerprinting and identification of the isolates were achieved by PCR with GTG₅ as well as 16S rRNA, phenylalanyl-tRNA synthetase alpha subunit (pheS), and RNA polymerase alpha subunit (rpoA) genes. This revealed the genomic diversity of the isolates from the two sources. Gene-specific amplification of probiotic marker genes was attained by PCR-based methods, and resultant products were sequenced. Multiple sequence alignment of the probiotic marker genes using bioinformatics revealed similarity to completely sequenced genomes of L. fermentum CECT 5716 and IFO 3956 with a few variations in mucin-binding protein gene sequences. Isolates designated as L. fermentum MCC 2759 and L. fermentum MCC 2760 showed the best probiotic attributes with high survival in simulated gastrointestinal fluids, in vitro adhesion, cholesterol reduction, and high antioxidative potential. Thus, these cultures could be potential probiotic candidates for application as functional foods. Keywords Probiotics L. fermentum Molecular fingerprinting Probiotic marker genes