Differential growth responses of Brachypodium distachyon genotypes to inoculation with plant growth promoting rhizobacteria

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• 作者：Fernanda P. do Amaral ; Vânia C. S. Pankievicz…
• 关键词：Brachypodium distachyon ; Plant ; beneficial bacteria interaction ; Azospirillum brasilense ; Herbaspirillum seropedicae
• 刊名：Plant Molecular Biology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：90
• 期：6
• 页码：689-697
• 全文大小：847 KB
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• 作者单位：Fernanda P. do Amaral (1)
  Vânia C. S. Pankievicz (2)
  Ana Carolina M. Arisi (3)
  Emanuel M. de Souza (2)
  Fabio Pedrosa (2)
  Gary Stacey (1)
  
  1. Divisions of Plant Science and Biochemistry, C. S. Bond Life Science Center, University of Missouri, Columbia, MO, 65211, USA
  2. Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, 81531-980, Brazil
  3. Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, 88034-001, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

Plant growth promoting rhizobacteria (PGPR) can associate and enhance the growth of important crop grasses. However, in most cases, the molecular mechanisms responsible for growth promotion are not known. Such research could benefit by the adoption of a grass model species that showed a positive response to bacterial inoculation and was amenable to genetic and molecular research methods. In this work we inoculated different genotypes of the model grass Brachypodium distachyon with two, well-characterized PGPR bacteria, Azospirillum brasilense and Herbaspirillum seropedicae, and evaluated the growth response. Plants were grown in soil under no nitrogen or with low nitrogen (i.e., 0.5 mM KNO₃). A variety of growth parameters (e.g., shoot height, root length, number of lateral roots, fresh and dry weight) were measured 35 days after inoculation. The data indicate that plant genotype plays a very important role in determining the plant response to PGPR inoculation. A positive growth response was observed with only four genotypes grown under no nitrogen and three genotypes tested under low nitrogen. However, in contrast, relatively good root colonization was seen with most genotypes, as measured by drop plate counting and direct, microscopic examination of roots. In particular, the endophytic bacteria H. seropedicae showed strong epiphytic and endophytic colonization of roots.