Biochemical Composition of Seminal Plasma and Annual Variations in Semen Characteristics of Jundi¨¢ Rhamdia quelen (Quoy and Gaimard, Pimelodidae)

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• 作者：Adriana Borges ; Daniele Rodrigues Siqueira ; Daiane Follmann Jurinitz ; Rebeca Zanini ; Fernanda do Amaral ; Marcelo Lacerda Grillo ; Eneder Rosana Oberst and Guillermo Federico Wassermann
• 关键词：seasonal changes ; semen ; seminal plasma ; silver catfish ; sperm motility ; spermatocrit ; spermatozoa
• 刊名：Fish Physiology and Biochemistry
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：31
• 期：1
• 页码：45-53
• 全文大小：278 KB

文摘

This study investigated the composition of milt of the South American silver catfish (Rhamdia quelen) or jundi¨¢. The semen was taken from jundi¨¢ in different periods during the four seasons. The biochemical composition of seminal fluid and the characteristics of sperm were analyzed. The semen quantity which can be extracted per fish in one day was 0.95 ¡À 0.08 ml during spring (maximum) and 0.24 ¡À 0.03 ml during winter (minimum). Sperm density (spermatocrit) showed higher values in the spring (75.1 ¡À 1.3 % ) decreasing slightly afterwards and reaching 63.0 ¡À 2.4 % to 65.0 ¡À 2.2 % in the fall and winter. Immediately after water dilution, 90–100 % of the spermatozoa presented vigorous straightforward motility that remained for at least 20 s. The total duration of the motility was 47.9 ¡À 1.3 s in the spring and 38.6 ¡À 0.6 s in the other seasons (P < 0.05). This pattern of motility is maintained for more than 2 h after storage of the milt at room temperature. The pH from 5 to 10 of the water dilution does not influence the sperm motility. The mean seminal pH and osmolality values were 8.7 ¡À 0.07 and 274.8 ¡À 11.2 (mOsm/kg), respectively. The ion concentration was: Na 153.7 ¡À 2.4, K 10.7 ¡À 2.4, Cl 139.4 ¡À 2.1, Ca 4.2 ¡À 0.2, Mg 0.9 ¡À 0.05, P 0.9 ¡À 0.08 (mEq/l). The total protein was 0.6 ¡À 0.05 mg/dl and cholesterol concentration was 13.9 ¡À 0.9 mg/dl.