Quantification of Blood Dendritic Cells in Colorectal Cancer Patients During the Course of Disease

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• 作者：Giulia Orsini (1)
  Annalisa Legitimo (1)
  Alessandra Failli (2)
  Paola Ferrari (1)
  Andrea Nicolini (1)
  Roberto Spisni (3)
  Paolo Miccoli (3)
  Rita Consolini (1)
  
• 关键词：Circulating dendritic cells ; Colorectal cancer ; Immunosuppression ; Flow cytometry
• 刊名：Pathology & Oncology Research
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：20
• 期：2
• 页码：267-276
• 全文大小：836 KB
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• 作者单位：Giulia Orsini (1)
  Annalisa Legitimo (1)
  Alessandra Failli (2)
  Paola Ferrari (1)
  Andrea Nicolini (1)
  Roberto Spisni (3)
  Paolo Miccoli (3)
  Rita Consolini (1)
  
  1. Department of Experimental and Clinical Medicine, University of Pisa, via Roma 67, Pisa, 56126, Italy
  2. Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy
  3. Department of Molecular, Medical and Surgical Pathology and of the Critical Area, University of Pisa, Pisa, Italy
  
• ISSN：1532-2807

文摘

Colorectal cancer is a malignancy with poor prognosis that might be associated with defective immune function. The aim of the present study was to investigate circulating dendritic cells in colorectal cancer patients, in order to contribute to elucidate tumor-escape mechanisms and to point out a possible correlation with the clinical condition of the disease. Therefore, we enumerated ex vivo myeloid and plasmacytoid dendritic cells, through multicolor flow cytometry, in 26 colorectal patients and 33 healthy controls. Furthermore we performed several analyses at determined time points in order to define the immunological trend of cancer patients after surgery and other conventional treatments. At the pre-operative time point the absolute number of plasmacytoid dendritic cells in cancer patients was significantly reduced in comparison to controls, this result being mainly referred to stage III-IV patients. The number of myeloid dendritic cells did not show any significant difference compared to healthy controls; interestingly the expression of the tolerogenic antigen CD85k was significantly higher on cancer patients-myeloid dendritic cells than controls- At the following samplings, circulating dendritic cell absolute number did not show any difference compared to controls. Conclusively the impairment of the number of circulating dendritic cells may represent one of the tumor escape mechanisms occurring in colorectal cancer. These alterations seem to be correlated to cancer progression. Our work sheds light on one of dendritic cell-based tumor immune escape mechanisms. This knowledge may be useful to the development of more effective immunotherapeutic strategies.