PIPKIIα is widely expressed in hematopoietic-derived cells and may play a role in the expression of alpha- and gamma-globins in K562 cells

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• 作者：Vania Peretti de Albuquerque Wobeto (1)
  Jo?o Agostinho Machado-Neto (2)
  Tania Regina Zaccariotto (1)
  Daniela Maria Ribeiro (1)
  Adriana da Silva Santos Duarte (2)
  Sara Teresinha Olalla Saad (2)
  Fernando Ferreira Costa (2)
  Maria de Fatima Sonati (1)
  
• 关键词：PIPKIIα ; Globins ; K562 cell ; Hematopoiesis ; Erythroid differentiation
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：393
• 期：1-2
• 页码：145-153
• 全文大小：2,531 KB
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• 作者单位：Vania Peretti de Albuquerque Wobeto (1)
  Jo?o Agostinho Machado-Neto (2)
  Tania Regina Zaccariotto (1)
  Daniela Maria Ribeiro (1)
  Adriana da Silva Santos Duarte (2)
  Sara Teresinha Olalla Saad (2)
  Fernando Ferreira Costa (2)
  Maria de Fatima Sonati (1)
  
  1. Department of Clinical Pathology, School of Medical Sciences, University of Campinas - UNICAMP, P.O. Box 6111, Campinas, S?o Paulo, 13083-970, Brazil
  2. Hematology and Hemotherapy Center, Instituto Nacional de Ciência e Tecnologia do Sangue, University of Campinas - UNICAMP, Campinas, S?o Paulo, Brazil
  
• ISSN：1573-4919

文摘

Characterized for the first time in erythrocytes, phosphatidylinositol phosphate kinases (PIP kinases) belong to a family of enzymes that generate various lipid messengers and participate in several cellular processes, including gene expression regulation. Recently, the PIPKIIα gene was found to be differentially expressed in reticulocytes from two siblings with hemoglobin H disease, suggesting a possible relationship between PIPKIIα and the production of globins. Here, we investigated PIPKIIα gene and protein expression and protein localization in hematopoietic-derived cells during their differentiation, and the effects of PIPKIIα silencing on K562 cells. PIPKIIα silencing resulted in an increase in α and γ globins and a decrease in the proliferation of K562 cells without affecting cell cycle progression and apoptosis. In conclusion, using a cell line model, we showed that PIPKIIα is widely expressed in hematopoietic-derived cells, is localized in their cytoplasm and nucleus, and is upregulated during erythroid differentiation. We also showed that PIPKIIα silencing can induce α and γ globin expression and decrease cell proliferation in K562 cells.