Redox-dependent activation of PI3-kinase is involved in growth-factor- induced proliferation of fibroblasts

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• 作者：P. A. Tyurin-Kuzmin ; N. D. Zhdanovskaya…
• 关键词：fibroblasts ; platelet ; derived growth factor ; proliferation ; hydrogen peroxide
• 刊名：Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：11
• 期：1
• 页码：17-23
• 全文大小：
• 刊物主题：Cell Biology;
• 出版者：Pleiades Publishing
• ISSN：1990-7494
• 卷排序：11

文摘

Proliferation of fibroblasts is important for wound healing and reparation. Platelet-derived growth factor (PDGF) is a key regulator of this activity in fibroblasts. Activation of the PDGF receptor is also associated with endogenous generation of hydrogen peroxide (H₂O₂). Here we studied the involvement of H₂O₂ in the proliferative response of fibroblasts. We found that PDGF increased cytoplasmic level of H₂O₂. This increase was blocked by apocynin, an inhibitor of the membrane assembly of NADPH oxidases. Apocynin significantly decreased PDGF-stimulated phosphorylation of Akt and the mitotic activity of fibroblasts. Apocynin did not affect the activaion of the Erk1/2 MAP-kinase pathway. These data testify in favor of the H₂O₂ effect specificity.