Characterization, tissue distribution, and expression of neuropeptide Y in olive flounder Paralichthys olivaceus

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• 作者：Qian Wang ; Xungang Tan …
• 关键词：neuropeptide Y ; brain ; fasting ; olive flounder ; feeding behavior ; Paralichthys olivaceus
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：33
• 期：3
• 页码：553-558
• 全文大小：365 KB
• 参考文献：Aldegunde M, Mancebo M. 2006. Effects of neuropeptide Y on food intake and brain biogenic amines in the rainbow trout (Oncorhynchus mykiss). Peptides, 27(4): 719-27.View Article
  Campos V F, Collares T, Deschamps J C, Seixas F K, Dellagostin O A, Lanes C F C, Sandrini J, Marins L F, Okamoto M, Sampaio L A, Robaldo R B. 2010. Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus. J. Biosci., 35(3): 405-13.View Article
  Carpio Y, Acosta J, Morales A, Herrera F, Gonzalez L J, Estrada M P. 2006. Cloning, expression and growth promoting action of Red tilapia (Oreochromis sp.) neuropeptide Y. Peptides, 27(4): 710-18.View Article
  Cerda-Reverter J M, Martinez-Rodriguez G, Zanuy S, Carrillo M, Larhammar D. 2000. Molecular evolution of the neuropeptide Y (NPY) family of peptides: cloning of three NPY-related peptides from the sea bass (Dicentrarchus labrax). Regul. Pept., 95(1-): 25-4.View Article
  Cerda-Reverter J M, Sorbera L A, Carrillo M, Zanuy S. 1999. Energetic dependence of NPY-induced LH secretion in a teleost fish (Dicentrarchus labrax). Am. J. Physiol. Regul. Integr. Comp. Physiol., 277(6): R1627–R1634.
  Drew R E, Rodnick K J, Settles M, Wacyk J, Churchill E, Powell M S, Hardy R W, Murdoch G K, Hill R A, Robison B D. 2008. Effect of starvation on transcriptomes of brain and liver in adult female zebrafish (Danio rerio). Physiol. Genomics, 35(3): 283-95.View Article
  Dumont Y, Martel J C, Fournier A, Stpierre S, Quirion R. 1992. Neuropeptide-Y and neuropeptide-Y receptor subtypes in brain and peripheral-tissues. Prog. Neurobiol., 38(2): 125-67.View Article
  Jones D T, Taylor W R, Thornton J M. 1992. The rapid generation of mutation data matrices from protein sequences. Comput. Appl. Biosci., 8: 275-82.
  Kehoe A S, Volkoff H. 2007. Cloning and characterization of neuropeptide Y (NPY) and cocaine and amphetamine regulated transcript (CART) in Atlantic cod (Gadus morhua). Comp. Biochem. Physiol. A-Mol. Integr. Physiol., 146(3): 451-61.View Article
  Kiris G A, Kumlu M, Dikel S. 2007. Stimulatory effects of neuropeptide Y on food intake and growth of Oreochromis niloticus. Aquaculture, 264(1-): 383-89.View Article
  Kurokawa T, Suzuki T. 2002. Development of neuropeptide Y-related peptides in the digestive organs during the larval stage of Japanese flounder, Paralichthys olivaceus. Gen. Comp. Endocrinol., 126(1): 30-8.View Article
  Larhammar D. 1996. Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide. Regul. Pept., 62(1): 1-1.View Article
  Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2Δοem class="EmphasisTypeItalic">Ct method. Methods, 25(4): 402-08.View Article
  Lopez-Patino M A, Guijarro A I, Isorna E, Delgado M J, Alonso-Bedate M, de Pedro N. 1999. Neuropeptide Y has a stimulatory action on feeding behavior in goldfish (Carassius auratus). Eur. J. Pharmacol., 377(2-): 147-53.View Article
  MacDonald E, Volkoff H. 2009. Neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and cholecystokinin (CCK) in winter skate (Raja ocellata): cDNA cloning, tissue distribution and mRNA expression responses to fasting. Gen. Comp. Endocrinol., 161(2): 252-61.View Article
  Murashita K, Kurokawa T, Ebbesson L O E, Stefansson S O, Ronnestad I. 2009. Characterization, tissue distribution, and regulation of agouti-related protein (AgRP), cocaine- and amphetamine-regulated transcript (CART) and neuropeptide Y (NPY) in Atlantic salmon (Salmo salar). Gen. Comp. Endocrinol., 162(2): 160-71.View Article
  Narnaware Y K, Peyon P P, Lin X W, Peter R E. 2000. Regulation of food intake by neuropeptide Y in goldfish. Am. J. Physiol.-Regul. Integr. Comp. Physiol., 279(3): R1025–R1034.
  Peng C, Chang J P, Yu K L, Wong A O L, Vangoor F, Peter R E, Rivier J E. 1993. Neuropeptide-Y stimulates growthhormone and gonadotropin-ii secretion in the goldfish pituitary: involvement of both presynaptic and pituitary cell actions. Endocrinology, 132(4): 1 820- 829.
  Silverstein J T, Plisetskaya E M. 2000. The effects of NPY and insulin on food intake regulation in fish. Am. Zool., 40(2): 296-08.View Article
  Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28(10): 2 731- 739.View Article
  Tang Z G, Sun C Y, Yan A F, Wu S G, Qin C B, Zhang Y H, Li W S. 2013. Genes involved in fatty acid metabolism: molecular characterization and hypothalamic mRNA response to energy status and neuropeptide Y treatment in the orange-spotted grouper Epinephelus coioides. Mol Cell Endocrinol., 376(1-): 114-24.View Article
  Tatemoto K. 1982. Neuropeptide-Y: complete amino-acidsequence of the brain peptide. Proc. Natl. Acad. Sci. USA, 79(18): 5
• 作者单位：Qian Wang (1) (2)
  Xungang Tan (1)
  Shaojun Du (3)
  Wei Sun (1)
  Feng You (1)
  Peijun Zhang (1)
  
  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  2. University of Chinese Academy of Sciences, Beijing, 100049, China
  3. Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 701 E. Pratt St, Baltimore, MD, 21202, USA
  
• 刊物主题：Oceanography;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5005

文摘

Neuropeptide Y (NPY) is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the h group before feeding, NPY expression in the 3 h group after feeding decreased significantly (P<0.05). Food deprivation led to an 81.7% decrease in NPY mRNA levels in the 24 h fasted group (P<0.05) and a 91.7% decrease in the 48 h fasted group (P<0.05). Therefore, our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture.