Tissue distribution of olive flounder VDAC2 and its expression in fish cell lines

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• 作者：Aijun Lü ; Xiucai Hu ; Li Li ; Chao Pei ; Chao Zhang…
• 关键词：VDAC ; Olive flounder ; Expression ; Fish cell lines
• 刊名：Fish Physiology and Biochemistry
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：41
• 期：4
• 页码：899-907
• 全文大小：2,708 KB
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• 作者单位：Aijun Lü (1) (2)
  Xiucai Hu (2)
  Li Li (1)
  Chao Pei (1)
  Chao Zhang (1)
  Xianglin Cao (1)
  Xianghui Kong (1)
  Guoxing Nie (1)
  Xuejun Li (1)
  Jingfeng Sun (3)
  
  1. College of Fisheries, Henan Normal University, Xinxiang, 453007, China
  2. School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China
  3. College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

Voltage-dependent anion channel (VDAC) is located in the mitochondrial outer membrane, which plays a crucial role in regulating cell life and death. In this study, the tissue distribution of olive flounder Paralichthys olivaceus VDAC2 (PoVDAC2) was detected by quantitative real-time PCR and Western blot analysis. The qRT-PCR results showed that the expression level of PoVDAC2 was abundant in heart, muscle and gill tissues. Western blot analysis revealed a protein of 32?kDa detected in all six tissues. Furthermore, a recombinant eukaryotic expression plasmid pEGFP-N3-PoVDAC2 was successfully constructed and transiently expressed the fusion protein in fish cell lines. Subcellular localization indicated that PoVDAC2-GFP was distributed in a punctate mitochondria-like pattern throughout the cytoplasm in flounder embryonic cells (FEC). The distribution of native VDAC2 in untransfected fish cells was also investigated by immunofluorescence microscopy. The punctate VDAC2 fluorescence signals of both FEC and EPC cells were identically observed in the cytoplasm but not in the nucleus. These results laid a foundation for investigating the functional relevance of VDAC response to pathogens in flounder.