Ectopic expression of a soybean SVP-like gene in tobacco causes abnormal floral organs and shortens the vegetative phase

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• 作者：Hengyou Zhang ; Honglang Yan ; Dan Zhang ; Deyue Yu
• 关键词：Soybean ; Floral organs ; Flowering time ; SHORT VEGETATIVE PHASE
• 刊名：Plant Growth Regulation
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：80
• 期：3
• 页码：345-353
• 全文大小：1,494 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087
• 卷排序：80

文摘

MADS-box family transcription factors play critical roles in regulating plant developmental processes. SHORT VEGETATIVE PHASE (SVP), a MADS-box family member, was demonstrated to be a key regulator in floral transition and identity in Arabidopsis thaliana. The present study isolated and characterized two soybean SVP-like genes, GmSVP1 and GmSVP2. A molecular sequence analysis demonstrated that each GmSVP protein contains a typical MADS_MEF2-like domain and a K-box region domain. GmSVPs are closely related to MtSVP and PsSVP but more distantly to AtSVP within the dicot SVP homology sub-clade. Quantitative real-time PCR assays showed intense expression of GmSVPs in floral organs, stamens and pistils during soybean flowering but no expression in soybean developing seeds or in the pod shell. In addition, GmSVP1 responded to abiotic stresses including low temperature and wounding. Ectopic expression of GmSVP1 caused the abnormal development of stamens, petals, and flower buds and slightly accelerated the flowering time in transgenic tobacco plants (Nicotiana tabacum). These results suggested that GmSVP1 acts as a regulator in soybean floral identity and flowering, which may be applied to the industrialization of hybrid soybean.