Calmodulin disruption impacts growth and motility in juvenile liver fluke

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• 作者：Erin M. McCammick ; Paul McVeigh ; Paul McCusker ; David J. Timson…
• 关键词：Fasciola hepatica ; Calmodulin ; Flukicide target ; RNAi ; Growth phenotype ; Motility phenotype
• 刊名：Parasites & Vectors
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：1
• 全文大小：1,916 KB
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• 作者单位：Erin M. McCammick (1)
  Paul McVeigh (1)
  Paul McCusker (1)
  David J. Timson (1)
  Russell M. Morphew (2)
  Peter M. Brophy (2)
  Nikki J. Marks (1)
  Angela Mousley (1)
  Aaron G. Maule (1)
  
  1. Microbes & Pathogen Biology: Institute for Global Food Security, School of Biological Sciences, Queen’s, University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK
  2. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, Ceredigion, SY23 3FL, UK
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background Deficiencies in effective flukicide options and growing issues with drug resistance make current strategies for liver fluke control unsustainable, thereby promoting the need to identify and validate new control targets in Fasciola spp. parasites. Calmodulins (CaMs) are small calcium-sensing proteins with ubiquitous expression in all eukaryotic organisms and generally use fluctuations in intracellular calcium levels to modulate cell signalling events. CaMs are essential for fundamental processes including the phosphorylation of protein kinases, gene transcription, calcium transport and smooth muscle contraction. In the blood fluke Schistosoma mansoni, calmodulins have been implicated in egg hatching, miracidial transformation and larval development. Previously, CaMs have been identified amongst liver fluke excretory-secretory products and three CaM-like proteins have been characterised biochemically from adult Fasciola hepatica, although their functions remain unknown.