An On-Column Enzyme Mediated Fluorescence-Amplification Method for Plasma Total Cholesterol Measurement by Capillary Electrophoresis with LIF Detection

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• 作者：Guobin Zhou ; Yueqing Guan
• 关键词：Total cholesterol ; Capillary electrophoresis (CE) ; On ; column enzymatic assay ; Laser ; induced fluorescence (LIF)
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：79
• 期：5-6
• 页码：319-325
• 全文大小：533 KB
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• 作者单位：Guobin Zhou (1)
  Yueqing Guan (2)
  
  1. Department of Chemistry, Taizhou University, Taizhou, 318000, China
  2. Department of Biological and Chemical Engineering, Taizhou Vocational and Technical College, Taizhou, 318000, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

Total cholesterol monitoring in human plasma is one of the most important routine analysis performed in the clinical laboratory. Here we report a new capillary electrophoresis method for determination of plasma total cholesterol based on on-column enzymatic assay and laser-induced fluorescence detection. In this method, the capillary is used not only as a separation medium but also as the reaction chamber which minimizes the amount of samples and the consumption of the reagents. The whole procedure can be automated which minimizes the possible errors and saves experimental time. The analytical characteristics of the proposed method, such as linearity and repeatability of the peak area and the migration time, were evaluated. The RSD of the migration time and peak area were 1.0 and 3.5 % (intraday), and 2.2 and 2.9 % (interday), respectively. The obtained LOQ values were at the nanomolar concentration level, therefore the developed method is sufficient for determination and quantification of plasma total cholesterol.