Light up Live Cell Nuclear Envelope in Real-Time Using a Two-Photon Absorption and AIE Chromophore

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• 作者：Xiaohe Tian ; Hui Wang ; Lijuan Guan ; Qiong Zhang ; Hongping Zhou…
• 关键词：Two ; photon absorption ; Aggregation induced emission ; Nuclear envelope ; Real ; time ; Live ; cell image
• 刊名：Journal of Fluorescence
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：26
• 期：1
• 页码：59-65
• 全文大小：931 KB
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• 作者单位：Xiaohe Tian (1) (2)
  Hui Wang (2)
  Lijuan Guan (3)
  Qiong Zhang (2)
  Hongping Zhou (2)
  Chunxia Li (1)
  Bei Huang (1)
  Jieying Wu (2)
  Yupeng Tian (2)
  
  1. School of Life Science, Anhui University, Hefei, 230601, People’s Republic of China
  2. Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People’s Republic of China
  3. Department of Chemistry, MRC Center for Medical Molecular Virology, University College London, London, WC1H 0AJ, UK
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biophysics and Biomedical Physics
  Biotechnology
  Biochemistry
  Analytical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4994

文摘

In this letter, aggregation from two-photon absorption (2PA) molecules in living cells were firstly observed and the related aggregation induced emission (AIE) properties were investigated as a cell tracer for L ((Z)-3-(4-(Bis(4-ethoxyphenyl) amino)phenyl)-2-(4-amino-phenyl)- acrylonitrile cyano-substituted ) based on triphenylamine with D–π–A model. L was further used as a two-photon absorption (2PA, λ_ex = 900, λ_em = 550 nm δ = 156 GM) live-cell marker for real-time, long-term cell growth and proliferation monitoring, with rapidly adhering whole intracellular membrane-rich system. Remarkably, different from existing organic AIE chromophores and other commercially available probes, L exhibited intense intracellular-AIE property with stable nuclear envelope (NE) staining under two-photon excited microscopy (TPEM) through detailed in cellulo studies. Keywords Two-photon absorption Aggregation induced emission Nuclear envelope Real-time Live-cell image