Single molecule DNA detection with an atomic vapor notch filter

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• 作者：Denis Uhland ; Torsten Rendler ; Matthias Widmann ; Sang-Yun Lee…
• 关键词：DNA detection ; fluorescence microscopy ; single molecules ; atomic filtering ; sodium spectroscopy
• 刊名：EPJ Quantum Technology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：2
• 期：1
• 全文大小：1802KB
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• 作者单位：Denis Uhland (1)
  Torsten Rendler (1)
  Matthias Widmann (1)
  Sang-Yun Lee (1)
  J枚rg Wrachtrup (1) (2)
  Ilja Gerhardt (1) (2)
  
  1. 3rd Physics Institute, University of Stuttgart and Stuttgart Research Center of Photonic Engineering (SCoPE) and IQST, Pfaffenwaldring 57, Stuttgart, 70569, Germany
  2. Max Planck Institute for Solid State Research, Heisenbergstra脽e 1, Stuttgart, 70569, Germany
  
• 刊物类别：Quantum Physics; Quantum Information Technology, Spintronics; Nanotechnology and Microengineering;
• 刊物主题：Quantum Physics; Quantum Information Technology, Spintronics; Nanotechnology and Microengineering;
• 出版者：Springer Berlin Heidelberg
• ISSN：2196-0763

文摘

The detection of single molecules has facilitated many advances in life- and material-science. Commonly the fluorescence of dye molecules is detected, which are attached to a non-fluorescent structure under study. For fluorescence microscopy one desires to maximize the detection efficiency together with an efficient suppression of undesired laser leakage. Here we present the use of the narrow-band filtering properties of hot atomic sodium vapor to selectively filter the excitation light from the red-shifted fluorescence of dye labeled single-stranded DNA molecules. A statistical analysis proves an enhancement in detection efficiency of more than 15% in a confocal and in a wide-field configuration. Keywords DNA detection fluorescence microscopy single molecules atomic filtering sodium spectroscopy