Seed-borne nature of a begomovirus, Mung bean yellow mosaic virus in black gram

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• 作者：Satya Vijayalakshmi Kothandaraman…
• 关键词：Begomovirus ; Seed ; borne viruses ; Black gram ; Legumes
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：100
• 期：4
• 页码：1925-1933
• 全文大小：1,434 KB
• 参考文献：Barnabas AD, Radhakrishnan GK, Usha R (2010) Characterization of a begomovirus causing horsegram yellow mosaic disease in India. Eur J Plant Pathol 127:41–51CrossRef
  Bennett CW (1936) Further studies on the relation of the curly top virus to plant tissues. J Agric Res 53:595–620
  Costa AS (1955) Studies of Abutilon mosaic in Brazil. Phytopathology 24:97–112
  de Silva DPP, Jones P, Shaw MW (2002) Identification and transmission of piper yellow mottle virus and cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri Lanka. Plant Pathol 51:537–545CrossRef
  Fauquet CM, Bisaro DM, Briddon RW, Brown J, Harrison BD, Rybicki EP, Stenger DC, Stanley J (2003) Revision of taxonomic criteria for species demarcation in the family Geminiviridae, and an updated list of begomovirus species. Arch Virol 148:405–421CrossRef PubMed
  Feinberg AP, Vogelstain B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Ann Biochem 132:6–13
  Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Vir Meth 135:9–16
  Hull R (2002) Matthews’s plant virology. Academic, New York
  Jakowitsch J, Mette MF, van der Winden J, Matzke MA, Matzke AJM (1999) Integrated pararetroviral sequences define a unique class of dispersed repetitive DNA in plants. Proc Natl Acad Sci U S A 96:13241–13246PubMedCentral CrossRef PubMed
  Keur JY (1934) Studies of the occurrence of and transmission of virus diseases in the genus Abutilon. Bull Torrey Bot Club 61:53–70CrossRef
  Kings AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2011) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego
  Macanawai A, Ebenebe HD, Devitt L, Hafner G, Harding R (2005) Investigations into the seed and mealybug transmission of Taro bacilliform virus. Aust J Plant Pathol 34:73–76CrossRef
  Malathi VG (2007) Genetic identity of yellow mosaic viruses infecting legumes and their phylogenetic relationship. Indian Phytopathol 60:143–155
  Maruthi MN, Rekha AR, Govindappa MR, Colvin J, Muniyappa V (2006) A distinct begomovirus causes Indian dolichos yellow mosaic disease. Plant Pathol 55:290
  Mink GI (1993) Pollen and seed transmitted viruses and viroids. Annu Rev Phytopathol 31:375–402CrossRef PubMed
  Moringa T, Ikegami M, Miura KI (1993) The nucleotide sequence and genome structure of mungbean yellow mosaic geminivirus. Microbiol Immunol 37:471–476CrossRef
  Naimuddin AM, Pratap A, Chaubey BK, John JK (2011) PCR based identification of the virus causing yellow mosaic disease in wild Vigna accessions. J Food Leg 24:14–17
  Nene YL (1973) Viral diseases of some warm weather pulse crops in India. Plant Dis Rep 57:463–467
  Nolan PA, Campbell RN (1984) Squash mosaic virus detection in individual seeds and seed lots of cucurbits by enzyme-linked immunosorbent assay. Plant Dis 68:971–975CrossRef
  Pahalawatta V, Druffel K, Pappu HR (2007) Seed transmission of Dahlia mosaic virus in Dahlia pinnata. Plant Dis 91:88–91CrossRef
  Paximadis M, Dusterwald G, Duyver L, Rey MEC (1997) Preliminary investigation of the causal agent(s) of a disease causing leaf curl of tobacco in South Africa. Plant Pathol 46:282–290CrossRef
  Quainoo AK, Wetten AC, Allainguillaume J (2008) Transmission of Cocoa swollen shoot virus by seeds. J Vir Meth 150:45–49CrossRef
  Rojas MR, Hagen C, Lucas WJ, Gilbertson RL (2005) Exploiting chinks in the plant’s armor: evolution and emergence of geminiviruses. Annu Rev Phytopathol 43:361–394CrossRef PubMed
  Rouhibakhsh A, Priya J, Periasamy M, Haq QMI, Malathi VG (2008) An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legume. J Vir Meth 147:37–42CrossRef
  Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual, vol I, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  Scott LM, Lockhart BEL, Olszewski NE (1992) The Commelina yellow mottle virus promoter is a strong promoter in vascular and reproductive tissues. Plant Cell 4:185–192CrossRef
  Thomson KG, Dietzgen RG, Thomas JE, Teakle DS (1996) Detection of pineapple bacilliform virus using the polymerase chain reaction. Ann Appl Biol 129:57–69CrossRef
  Usharani KS, Surendranath B, Haq QMR, Malathi VG (2004) Yellow mosaic virus infecting soybean in Northern India is distinct from the species infecting soybean in Southern and Western India. Curr Sci 86:845–850
  Varma A, Dhar AK, Mandal B (1992) MYMV transmission and control in India. In: Green SK, Kim DH (eds) Mungbean yellow mosaic disease. Proceedings of an International Workshop, Bangkok 1991. Publication No. 92–373 AVRDC, Shanhua, Tainan, Taiwan. pp 8–27
  Wang D, Maule AJ (1994) A model for seed transmission of a plant virus: genetic and structural analyses of a pea embryo invasion by Pea seed-borne mosaic virus. Plant Cell 6:777–787PubMedCentral CrossRef PubMed
  
• 作者单位：Satya Vijayalakshmi Kothandaraman (1)
  Alice Devadason (2)
  Malathi Varagur Ganesan (2)
  
  1. National Pulses Research Centre, Vamban, Pudukottai, Tamil Nadu, 622303, India
  2. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641001, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The yellow mosaic viruses (YMV) infecting legumes are considered to be the most devastating begomoviruses as they incite considerable yield loss. The yellow discoloration of pods and seeds of infected plants and symptom emergence in the very first trifoliate leaf of the plants in the field were suggestive that the virus may be seed borne, which was investigated in the present study. The distribution of the virus in various parts of the seeds of black gram (Vigna mungo L. Hepper) plants naturally infected in the field was determined by polymerase chain reaction (PCR), Southern blot analysis, and sequencing. Nucleotide sequencing of the PCR amplicons from the seed parts from groups of ten seeds revealed the presence of mung bean yellow mosaic virus (MYMV) in the seed coat, cotyledon, and embryonic axes. The presence of virion particles was confirmed through double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunosorbent electron microscopy (ISEM) even in a single whole seed. In confocal microscopy, positive fluorescent signals were obtained using coat protein gene-specific primers in the embryonic axes. However, in the growth tests performed with the same batch of seeds, there was no symptom development in the seedlings though the virus (both DNA A and B components) was detected in 32 % of tested seedlings. In this study, the MYMV was detected in seed coat, cotyledon, and embryo. This study revealed that the MYMV is a seed-borne virus. Keywords Begomovirus Seed-borne viruses Black gram Legumes