Effect of different proteins on reabsorption of yellow fluorescent protein in the kidney of the brown frog Rana temporaria

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• 作者：N. P. Prutskova ; E. V. Seliverstova
• 刊名：Journal of Evolutionary Biochemistry and Physiology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：52
• 期：3
• 页码：246-251
• 全文大小：251 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Evolutionary Biology
  Biochemistry
  Animal Physiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3202
• 卷排序：52

文摘

Protein reabsorption in the proximal tubules (PT) of the frog kidney was studied by immunohistochemistry, fluorescent and confocal microscopy. The yellow fluorescent protein (YFP) was introduced in combination with other proteins. Reabsorption of YFP co-injected with lysozyme or the green fluorescent protein (GFP) was indistinguishable from that of YFP injection alone. Preliminary lysozyme injection did not change YFP absorption in contrast to YFP uptake reduced after GFP pretreatment. Lysozyme loading for 4 days led to a significant reduction in YFP absorption. The results show that receptor-mediated endocytosis in the frog kidney depends on the molecular nature of absorbable ligands, conditions of their competitive absorption and lysosomal accumulation in PT epithelial cells.Key wordsfrog kidneyproximal tubuleprotein reabsorptionlysozymefluorescent protein