Folate, Vitamin B12 and Homocysteine status in the post-folic acid fortification era in different subgroups of the Brazilian population attended to at a public health care center

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• 作者：Aline Barnabé ; Ana Cláudia Morandi Aléssio ; Luis Fernando Bittar…
• 关键词：Folate ; Vitamin B12 ; Homocysteine ; Folic acid fortification ; Reduced folate carrier ; Glutamate carboxypeptidase II ; Methylenetetrahydrofolate reductase
• 刊名：Nutrition Journal
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：14
• 期：1
• 全文大小：404 KB
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  Homocysteine and risk of ischemic heart disease and stroke: a meta-analysis. Jama 288: pp. 2015-22 CrossRef
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  11. Ministério da Saúde, Agência Nacional de Vigilancia Sanitária do Brasil (ANVISA). In: Resolu??o RDC n° 344. 2002. http://portal.anvisa.gov.br/wps/wcm/connect/f851a500474580668c83dc3fbc4c6735/RDC_344_2002.pdf?MOD=AJPERES. Accessed 18 Jun 2013.
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nutrition
  Clinical Nutrition
  
• 出版者：BioMed Central
• ISSN：1475-2891

文摘

Background Folate and vitamin B12 are essential nutrients, whose deficiencies are considerable public health problems worldwide, affecting all age groups. Low levels of these vitamins have been associated with high concentrations of homocysteine (Hcy) and can lead to health complications. Several genetic polymorphisms affect the metabolism of these vitamins. The aims of this study were to assess folate, vitamin B12 and homocysteine status in distinct Brazilian individuals after the initiation of folic acid fortification by Brazilian authorities and to investigate the effects of RFC1 A80G, GCPII C1561T and MTHFR C677T polymorphisms on folate, vitamin B12 and Hcy levels in these populations. Methods A total of 719 individuals including the elderly, children, as well as pregnant and lactating women were recruited from our health care center. Folate, vitamin B12 and Hcy levels were measured by conventional methods. Genotype analyses of RFC1 A80G, GCPII C1561T and MTHFR C677T polymorphisms were performed by PCR-RFLP. Results The overall prevalence of folate and vitamin B12 deficiencies were 0.3% and 4.9%, respectively. Folate deficiency was observed only in the elderly (0.4%) and pregnant women (0.3%), whereas vitamin B12 deficiency was observed mainly in pregnant women (7.9%) and the elderly (4.2%). Plasma Hcy concentrations were significantly higher in the elderly (33.6%). Pregnant women carrying the MTHFR 677TT genotype showed lower serum folate levels (p--.042) and higher Hcy levels (p--.003). RFC1 A80G and GCPII C1561T polymorphisms did not affect folate and Hcy levels in the study group. After a multivariate analysis, Hcy levels were predicted by variables such as folate, vitamin B12, gender, age and RFC1 A80G polymorphism, according to the groups studied. Conclusion Our results suggest that folate deficiency is practically nonexistent in the post-folic acid fortification era in the subgroups evaluated. However, screening for vitamin B12 deficiency may be particularly relevant in our population, especially in the elderly.