Diadenosine tetraphosphate contributes to carbachol-induced tear secretion

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• 作者：Bego?a Fonseca ; Alejandro Martínez-águila ; Miguel Díaz-Hernández…
• 关键词：Carbachol ; Purinergic ; Nucleotides ; Tear secretion ; P2Y
• 刊名：Purinergic Signalling
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：11
• 期：1
• 页码：87-93
• 全文大小：520 KB
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• 作者单位：Bego?a Fonseca (1)
  Alejandro Martínez-águila (1)
  Miguel Díaz-Hernández (2)
  Jesús Pintor (1)
  
  1. Dpto de Bioquímica y Biología Molecular IV, Facultad de óptica y Optometría, Universidad Complutense de Madrid, C/ Arcos de Jalón, 118, E-28037, Madrid, Spain
  2. Dpto de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Pharmacology and Toxicology
  Human Physiology
  Neurosciences
  Cancer Research
  
• 出版者：Springer Netherlands
• ISSN：1573-9546

文摘

The purpose of this study is to investigate if the cholinergic stimulation by carbachol on tear secretion is a direct process or if it is also mediated by purinergic mechanisms. Experiments were performed in New Zealand male rabbits. The amount of tear secretion was measured with Schirmer’s test and then analyzed by a HPLC protocol in order to study the nucleotide levels. Animal eyes were instilled with carbachol (a cholinergic agonist), pirenzepine, gallamine and 4-DAMP (muscarinic antagonists), PPADS, suramin and reactive blue 2 (purinergic antagonists), and a P2Y2 receptor small interfering RNA (siRNA). Tear secretion increased with the instillation of carbachol, approximately 84?% over control values 20?min after the instillation and so did Ap4A and ATP release. When we applied carbachol in the presence of muscarinic antagonists, tear volume only increased to 4?% with atropine, 12?% in the case of pirenzepine, 3?% with gallamine, and 8?% with 4-DAMP. In the presence of carbachol and purinergic antagonists, tear secretion was increased to 12?% (all values compared to basal tear secretion). By analyzing tear secretion induced with carbachol in presence of a P2Y2 receptor siRNA, we found that tear secretion was diminished to 60?%. The inhibition of tear secretion in the presence of carbachol and purinergic antagonists or P2Y2 siRNA occurred with no apparent change in the tear amount of Ap4A. These experiments demonstrated the participation of Ap4A in lacrimal secretion process.