Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells
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- 作者：Akihiro Hosoya (1) hosoya@po.mdu.ac.jp
Toru Hiraga (1)
Tadashi Ninomiya (2)
Akira Yukita (1)
Kunihiko Yoshiba (3)
Nagako Yoshiba (3)
Masafumi Takahashi (4)
Susumu Ito (5)
Hiroaki Nakamura (1) - 关键词：Subodontoblastic cell ; rich layer – ; Thy ; 1 (CD90) – ; Odontoblast progenitor – ; Dental pulp – ; Matrix proteins
- 刊名：Histochemistry and Cell Biology
- 出版年：2012
- 出版时间：June 2012
- 年：2012
- 卷：137
- 期：6
- 页码：733-742
- 全文大小：927.1 KB
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- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Anatomy
Medicine/Public Health, general - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-119X
文摘
The cells of the subodontoblastic cell-rich layer in dental pulp are speculated to contain odontoblast progenitor cells because of their positional relationship with odontoblasts as well as their high alkaline phosphatase (ALP) activity. However, it has yet to be determined whether these cells have the ability to differentiate into odontoblastic cells. In the present study, we firstly found that the majority of cells in the subodontoblastic layer expressed Thy-1, a cell-surface marker of stem and progenitor cells. Then, we evaluated the capacity of Thy-1 high- and low-expressing (Thy-1high and Thy-1low) cells separated from rat dental pulp cells by use of a fluorescence-activated cell sorter to differentiate into hard tissue-forming cells in vitro and in vivo. Following stimulation with bone morphogenetic protein-2, Thy-1high cells in vitro showed accelerated induction of ALP activity and formation of alizarin red-positive mineralized matrix compared with Thy-1low cells. Furthermore, subcutaneous implantation of Thy-1high cells efficiently induced the formation of bone-like matrix. These results collectively suggest that Thy-1-positive dental pulp cells localized in the subodontoblastic layer had the ability to differentiate into hard tissue-forming cells, and thus these cells may serve as a source of odontoblastic cells.