Renal tubular injury induced by ischemia promotes the formation of calcium oxalate crystals in rats with hyperoxaluria
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- 作者：Yanwei Cao ; Wanpeng Liu ; Limei Hui ; Jianjun Zhao ; Xuecheng Yang…
- 关键词：Renal dysfunction ; Urolithiasis ; Calcium oxalate ; Ischemia
- 刊名：Urological Research
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：44
- 期：5
- 页码：389-397
- 全文大小：2,243 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Urology and Andrology
Nephrology
Medical Biochemistry - 出版者：Springer Berlin / Heidelberg
- ISSN：1434-0879
- 卷排序：44
文摘
Hyperoxaluria and cell injury are key factors in urolithiasis. Oxalate metabolism may be altered by renal dysfunction and therefore, impact the deposition of calcium oxalate (CaOx) crystals. We investigated the relationship of renal function, oxalate metabolism and CaOx crystal deposition in renal ischemia. One hundred male Sprague–Dawley rats were randomly divided into four groups. Hyperoxaluria model (Group A and B) was established by feeding rats with 0.75 % ethylene glycol (EG). The left renal pedicle was clamped for 30 min to establish renal ischemia Groups (B and C), while Groups A and D underwent sham operation. Then, serum and urine oxalate (Ox), creatinine (Cr) and urea nitrogen (UN) levels were evaluated by liquid chromatography mass spectrometry (LCMS) and ion mass spectrum (IMS) at days 0, 2, 4, 7, and 14. CaOx crystallization was assessed by transmission electron microscope (TEM). A temporal and significant increase of serum Cr and UN levels was observed in Groups B and C compared to values obtained for Groups A and D (P < 0.05). Ox levels in serum and urine were significantly higher in Groups A and B than in the other two groups from day 7 (P < 0.05). In addition, CaOx crystallization was observed in both Groups A and B, but Group B showed earlier and more pronounced crystal deposition in the renal tissue. Our results indicated that renal tubular injury induced by renal ischemia might not affect Ox levels but could promote CaOx crystal retention under hyperoxaluria.