Pharmacokinetic study of unbound forsythiaside in rat blood and bile by microdialysis coupled with HPLC method

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• 作者：Yang Chu (1)
  Xiangyang Wang (1)
  Jiahua Guo (1)
  Wei Li (1)
  Xiaohui Ma (1)
  Yonghong Zhu (1)
  
• 关键词：Forsythiaside ; Microdialysis ; HPLC ; Pharmacokinetics ; Hepatobiliary excretion
• 刊名：European Journal of Drug Metabolism and Pharmacokinetics
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：37
• 期：3
• 页码：173-177
• 全文大小：338KB
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• 作者单位：Yang Chu (1)
  Xiangyang Wang (1)
  Jiahua Guo (1)
  Wei Li (1)
  Xiaohui Ma (1)
  Yonghong Zhu (1)
  
  1. Department of Pharmacology, Tianjin Tasly Institute, No.2, Pujihe East Road, Beichen District, Tianjin, 300410, People’s Republic of China
  

文摘

In the present study, an in vivo microdialysis sampling method coupled to HPLC was applied for the determination of unbound forsythiaside in rat blood and bile. Microdialysis probes were inserted into the jugular vein and bile duct of rats, and then blood and bile dialysates were collected at regular time intervals after intravenous administration of forsythiaside (50?mg/kg). Dialysates were directly injected into HPLC system. Forsythiaside was separated on a C18 column eluted with the mobile phase of acetonitrile–water–formic acid (16:84:0.2, v/v/v) at a flow rate of 0.8?mL/min. The wavelength of the ultraviolet detector was set at 332?nm. The lowest limit of quantification was 0.2?μg/mL for forsythiaside. Excellent linearity was found to be over a concentration range of 0.2-00?μg/mL. The main pharmacokinetic parameters of unbound forsythiaside in rat blood and bile were obtained. Furthermore, the bile-to-blood distribution ratio (AUCbile/AUCblood) of forsythiaside was 0.32?±?0.06. The results indicated that forsythiaside went through hepatobiliary excretion.