Chitosan Degradation Products Promote Nerve Regeneration by Stimulating Schwann Cell Proliferation via miR-27a/FOXO1 Axis

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• 作者：Yongjun Wang ; Yahong Zhao ; Cheng Sun ; Wen Hu ; Jing Zhao…
• 关键词：Biomaterials ; Polysaccharides ; miRNA ; Oligosaccharides ; Regeneration
• 刊名：Molecular Neurobiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：53
• 期：1
• 页码：28-39
• 全文大小：4,731 KB
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• 作者单位：Yongjun Wang (1) (2)
  Yahong Zhao (1) (2)
  Cheng Sun (1) (2)
  Wen Hu (1) (2)
  Jing Zhao (1) (2)
  Guicai Li (1) (2)
  Luzhong Zhang (1) (2)
  Mei Liu (1) (2)
  Yan Liu (1) (2)
  Fei Ding (1) (2)
  Yumin Yang (1) (2)
  Xiaosong Gu (1) (2)
  
  1. Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, 226001, People’s Republic of China
  2. Co-innovation Center of Neuroregeneration, Nantong, 226001, Jiangsu Province, People’s Republic of China
  
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182

文摘

Natural polysaccharides are biomaterials widely used for constructing scaffolds in tissue engineering. While natural polysaccharides have been shown to robustly promote tissue regeneration, the underlying molecular mechanism remains largely unknown. Here, we show that chitooligosaccharides (COS), the intermediate products of chitosan degradation, stimulate peripheral nerve regeneration in rats. Our experiment also shows that COS stimulate the proliferation of Schwann cells (SCs) during nerve regeneration. By analyzing the transcriptome and gene regulatory network, we identified the miR-27a/FOXO1 axis as the main signaling pathway for mediating the proliferative effects of COS on SCs. COS increase the expression level of miR-27a and cause a reduction of FOXO1, which subsequently accelerates the cell cycle and stimulates SC proliferation to stimulate nerve regeneration. These findings define a basic pathway for oligosaccharides-mediated cell proliferation and reveal a novel aspect of polysaccharide biomaterials in tissue engineering.