Quantifying growing versus non-growing ovarian follicles in the mouse
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- 作者：Bahar Uslu ; Carola Conca Dioguardi ; Monique Haynes…
- 关键词：Atresia ; Follicle ; Fragile X ; Histomorphometric evaluation ; Oocyte ; Ovarian reserve ; Ovary ; Premutation
- 刊名：Journal of Ovarian Research
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：4589KB
- 刊物主题：Gynecology; Reproductive Medicine;
- 出版者：BioMed Central
- ISSN：1757-2215
- 卷排序：10
文摘
BackgroundA standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle, it should be categorized as atretic. Many studies have used these criteria to estimate the fraction of atretic follicles that result from genetic manipulation or environmental insult. During an analysis of follicle development in a mouse model of Fragile X premutation, we asked whether these ‘historical’ criteria could correctly identify follicles that were not growing (and could thus confirmed to be dying).