Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

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• 作者：María Pilar Castro-Gómez ; Francisca Holgado…
• 关键词：Fatty acid ; Krill oil ; Lipid classes ; Phospholipid ; Chromatography techniques
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：8
• 期：10
• 页码：2568-2580
• 全文大小：385 KB
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• 作者单位：María Pilar Castro-Gómez (1)
  Francisca Holgado (1)
  Luis Miguel Rodríguez-Alcalá (1)
  Olimpio Montero (2)
  Javier Fontecha (1)
  
  1. Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Universidad Autónoma de Madrid, Calle Nicolás Cabrera 9, 28049, Madrid, Spain
  2. Centre for Biotechnology Development (CDB, CSIC), Boecillo Technological Park, 47151, Valladolid, Spain
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

Krill oil represents an interesting source of bioactive lipid components, being suitable as a functional ingredient. This oil is characterized by its high concentration of long-chain omega-3 fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The contents of EPA and DHA were similar to those in fish oils, but with the difference that almost the half are located in phospholipids (mainly phosphatidylcholine). This might explain its higher absorption and bioavailability. This highly unsaturated oil maintains stable due to the presence of astaxanthin, a potent antioxidant, which assures the stability of the omega-3 fatty acids. However, there is lack of investigations reporting a deep comprehensive description of the krill oil (KO) lipid composition. The characterization includes new data of its neutral and polar components and the identification of triacylglycerols, diacylglycerols, and molecular species that has been done by different chromatographic techniques as gas chromatography–mass spectrometry/flame ionization detector (GC-MS/FID), flash chromatography–evaporative light scattering detector (FC-ELSD), and HPLC-ELSD. Also phospholipid molecular species by using ultraperformance liquid chromatography/quadruple-time-of-flight mass spectrometry (UPLC/QToF-MS) have been determined. Keywords Fatty acid Krill oil Lipid classes Phospholipid Chromatography techniques