Development and characterization of a synthetic infectious cDNA clone of the virulent Bucyrus strain of equine arteritis virus expressing mCherry (red fluorescent protein)

详细信息    查看全文

• 作者：Shankar P. Mondal ; R. Frank Cook ; R. Lakshman Chelvarajan…
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：161
• 期：4
• 页码：821-832
• 全文大小：3,133 KB
• 参考文献：1.Balasuriya UB, Rossitto PV, DeMaula CD, MacLachlan NJ (1993) A 29K envelope glycoprotein of equine arteritis virus expresses neutralization determinants recognized by murine monoclonal antibodies. J Gen Virol 74(Pt 11):2525–2529CrossRef PubMed
  2.Balasuriya UB, Maclachlan NJ, De Vries AA, Rossitto PV, Rottier PJ (1995) Identification of a neutralization site in the major envelope glycoprotein (GL) of equine arteritis virus. Virology 207:518–527CrossRef PubMed
  3.Balasuriya UB, Evermann JF, Hedges JF, McKeirnan AJ, Mitten JQ, Beyer JC, McCollum WH, Timoney PJ, MacLachlan NJ (1998) Serologic and molecular characterization of an abortigenic strain of equine arteritis virus isolated from infective frozen semen and an aborted equine fetus. J Am Vet Med Assoc 213:1586-1589, 1570
  4.Balasuriya UB, Snijder EJ, van Dinten LC, Heidner HW, Wilson WD, Hedges JF, Hullinger PJ, MacLachlan NJ (1999) Equine arteritis virus derived from an infectious cDNA clone is attenuated and genetically stable in infected stallions. Virology 260:201–208CrossRef PubMed
  5.Balasuriya UB, Heidner HW, Davis NL, Wagner HM, Hullinger PJ, Hedges JF, Williams JC, Johnston RE, David Wilson W, Liu IK, James MacLachlan N (2002) Alphavirus replicon particles expressing the two major envelope proteins of equine arteritis virus induce high level protection against challenge with virulent virus in vaccinated horses. Vaccine 20:1609–1617CrossRef PubMed
  6.Balasuriya UB, Snijder EJ, Heidner HW, Zhang J, Zevenhoven-Dobbe JC, Boone JD, McCollum WH, Timoney PJ, MacLachlan NJ (2007) Development and characterization of an infectious cDNA clone of the virulent Bucyrus strain of Equine arteritis virus. J Gen Virol 88:918–924CrossRef PubMed
  7.Balasuriya UB, Go YY, Maclachlan NJ (2013) Equine arteritis virus. Vet Microbiol 167:93–122CrossRef PubMed
  8.Balasuriya UB (2014) Equine viral arteritis. Vet Clin N Am Equine Pract 30:543–560CrossRef
  9.Balasuriya UB, Zhang J, Go YY, MacLachlan NJ (2014) Experiences with infectious cDNA clones of equine arteritis virus: lessons learned and insights gained. Virology 462–463:388–403CrossRef PubMed
  10.Boyer J-C, Haenni A-L (1994) Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415–426CrossRef PubMed
  11.Bryans JT, Crowe ME, Doll ER, McCollum WH (1957) The blood picture and thermal reaction in experimental viral arteritis of horses. Cornell Vet 47:42–52PubMed
  12.Cavanagh D (1997) Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142:629–633PubMed
  13.de Vries AA, Glaser AL, Raamsman MJ, Rottier PJ (2001) Recombinant equine arteritis virus as an expression vector. Virology 284:259–276CrossRef PubMed
  14.Firth AE, Zevenhoven-Dobbe JC, Wills NM, Go YY, Balasuriya UB, Atkins JF, Snijder EJ, Posthuma CC (2011) Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production. J Gen Virol 92:1097–1106CrossRef PubMed PubMedCentral
  15.Go YY, Zhang J, Timoney PJ, Cook RF, Horohov DW, Balasuriya UB (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol 84:4898–4911CrossRef PubMed PubMedCentral
  16.Go YY, Bailey E, Cook DG, Coleman SJ, Macleod JN, Chen KC, Timoney PJ, Balasuriya UB (2011) Genome-wide association study among four horse breeds identifies a common haplotype associated with in vitro CD3+ T cell susceptibility/resistance to equine arteritis virus infection. J Virol 85:13174–13184CrossRef PubMed PubMedCentral
  17.Go YY, Bailey E, Timoney PJ, Shuck KM, Balasuriya UB (2012) Evidence that in vitro susceptibility of CD3+ T lymphocytes to equine arteritis virus infection reflects genetic predisposition of naturally infected stallions to become carriers of the virus. J Virol 86:12407–12410CrossRef PubMed PubMedCentral
  18.Hedges JF, Balasuriya UB, Topol JB, Lee DW, MacLachlan NJ (2001) Genetic variation of ORFs 3 and 4 of equine arteritis virus. Adv Exp Med Biol 494:69–72CrossRef PubMed
  19.Johnson CR, Griggs TF, Gnanandarajah J, Murtaugh MP (2011) Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses. The Journal of general virology 92:1107–1116CrossRef PubMed PubMedCentral
  20.MacLachlan NJ, Balasuriya UB, Rossitto PV, Hullinger PA, Patton JF, Wilson WD (1996) Fatal experimental equine arteritis virus infection of a pregnant mare: immunohistochemical staining of viral antigens. J Vet Diagn Invest 8:367–374CrossRef PubMed
  21.McCollum WH, Prickett ME, Bryans JT (1971) Temporal distribution of equine arteritis virus in respiratory mucosa, tissues and body fluids of horses infected by inhalation. Res Vet Sci 12:459–464PubMed
  22.McCollum WH, Timoney PJ (1998) Experimental observations on the virulence of isolates of equine arteritis virus. In: Proceedings of the 8th international conference equine infectious diseases, Dubai. R & W Publications (Newmarket) Limited, pp 558–559
  23.McCollum WH, Doll ER, Wilson JC, Cheatham J (1962) Isolation and propagation of equine arteritis virus in monolayer cell cultures of rabbit kidney. Cornell Vet 52:452–458
  24.Moore BD, Balasuriya UB, Hedges JF, MacLachlan NJ (2002) Growth characteristics of a highly virulent, a moderately virulent, and an avirulent strain of equine arteritis virus in primary equine endothelial cells are predictive of their virulence to horses. Virology 298:39–44CrossRef PubMed
  25.Moore BD, Balasuriya UB, Nurton JP, McCollum WH, Timoney PJ, Guthrie AJ, MacLachlan NJ (2003) Differentiation of strains of equine arteritis virus of differing virulence to horses by growth in equine endothelial cells. Am J Vet Res 64:779–784CrossRef PubMed
  26.Reed LJ, Muench H (1938) A simple method of estimating fifty percent end points. Am J Hyg 27:493–497
  27.Snijder EJ, Wassenaar AL, Spaan WJ (1992) The 5′ end of the equine arteritis virus replicase gene encodes a papainlike cysteine protease. J Virol 66:7040–7048PubMed PubMedCentral
  28.Snijder EJ, van Tol H, Pedersen KW, Raamsman MJ, de Vries AA (1999) Identification of a novel structural protein of arteriviruses. J Virol 73:6335–6345PubMed PubMedCentral
  29.Snijder EJ, Kikkert M, Fang Y (2013) Arterivirus molecular biology and pathogenesis. J Gen Virol 94:2141–2163CrossRef PubMed
  30.van Aken D, Benckhuijsen WE, Drijfhout JW, Wassenaar AL, Gorbalenya AE, Snijder EJ (2006) Expression, purification, and in vitro activity of an arterivirus main proteinase. Virus Res 120:97–106CrossRef PubMed
  31.van den Born E, Posthuma CC, Gultyaev AP, Snijder EJ (2005) Discontinuous subgenomic RNA synthesis in arteriviruses is guided by an RNA hairpin structure located in the genomic leader region. J Virol 79:6312–6324CrossRef PubMed PubMedCentral
  32.van den Born E, Posthuma CC, Knoops K, Snijder EJ (2007) An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J Gen Virol 88:1196–1205CrossRef PubMed
  33.van Dinten LC, den Boon JA, Wassenaar AL, Spaan WJ, Snijder EJ (1997) An infectious arterivirus cDNA clone: identification of a replicase point mutation that abolishes discontinuous mRNA transcription. Proc Natl Acad Sci USA 94:991–996CrossRef PubMed PubMedCentral
  34.Villefranc JA, Amigo J, Lawson ND (2007) Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn 236:3077–3087CrossRef PubMed PubMedCentral
  35.Wagner HM, Balasuriya UB, James MacLachlan N (2003) The serologic response of horses to equine arteritis virus as determined by competitive enzyme-linked immunosorbent assays (c-ELISAs) to structural and non-structural viral proteins. Comp Immunol Microbiol Infect Dis 26:251–260CrossRef PubMed
  36.Zhang J, Timoney PJ, MacLachlan NJ, McCollum WH, Balasuriya UB (2008) Persistent equine arteritis virus infection in HeLa cells. J Virol 82:8456–8464CrossRef PubMed PubMedCentral
  37.Ziebuhr J, Snijder EJ, Gorbalenya AE (2000) Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 81:853–879CrossRef PubMed
  
• 作者单位：Shankar P. Mondal (1)
  R. Frank Cook (1)
  R. Lakshman Chelvarajan (1)
  Pamela J. Henney (1)
  Peter J. Timoney (1)
  Udeni B. R. Balasuriya (1)
  
  1. 108 Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, 40546-0099, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Strains of equine arteritis virus (EAV) differ in their virulence phenotypes, causing anywhere from subclinical infections to severe disease in horses. Here, we describe the in silico design and de novo synthesis of a full-length infectious cDNA clone of the horse-adapted virulent Bucyrus strain (VBS) of EAV encoding mCherry along with in vitro characterization of the progeny virions (EAV sVBSmCherry) in terms of host-cell tropism, replicative capacity and stability of the mCherry coding sequences following sequential passage in cell culture. The relative stability of the mCherry sequence during sequential cell culture passage coupled with a comparable host-cell range phenotype (equine endothelial cells, CD3+ T cells and CD14+ monocytes) to parental EAV VBS suggest that EAV-sVBSmCherry-derived virus could become a valuable research tool for identification of host-cell tropism determinants and for characterization of the viral proteins involved in virus attachment and entry into different subpopulations of peripheral blood mononuclear cells. Furthermore, this study demonstrates that advances in nucleic acid synthesis technology permit synthesis of complex viral genomes with overlapping genes like those of arteriviruses, thereby circumventing the need for complicated molecular cloning techniques. In summary, de novo nucleic acid synthesis technology facilitates innovative viral vector design without the tedium and risks posed by more-conventional laboratory techniques.