Letter to the Editor: Structure of the hypothetical protein At3g17210 from Arabidopsis thaliana *
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- 作者：Lytle ; Betsy L. ; Peterson ; Francis C. ; Kjer ; Kelly L. ; Frederick ; Ronnie O. ; Zhao ; Qin ; Thao ; Sandy ; Bingman ; Craig ; Johnson ; Kenneth A. ; Phillips ; Jr. ; George N. ; Volkman ; Brian F.
- 关键词：automated refinement ; high ; throughput ; NMR ; structural genomics
- 刊名：Journal of Biomolecular NMR
- 出版年：2004
- 出版时间：2004
- 年：2004
- 卷：28
- 期：4
- 页码：397-400
- 全文大小：309 KB
文摘
Four-dimensional NMR spectroscopy can be speeded up by a large factor by a projection-reconstruction technique related to that used in X-ray tomography. The limited amount of information recorded in a few suitably-tilted projection planes suffices to recreate the entire four-dimensional spectrum. The method is demonstrated by reference to the 500 MHz spectrum of 1 millimolar aqueous solution of ubiquitin by dispersing the responses of the three-dimensional HNCO spectrum into the C&agr; dimension of a four-dimensional HNCOCA spectrum obtained by projection-reconstruction.