Immobilization of Fusarium graminearum β-d-fructofuranosidase using alternative cellulosic supports: Stabilization and production of fructooligosaccharides

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• 作者：Heloísa Bressan Gon?alves ; Jo?o Atílio Jorge…
• 关键词：enzyme immobilization ; enzyme stabilization ; Fusarium graminearum ; fructooligosaccharides ; β ; d ; fructofuranosidase
• 刊名：Food Science and Biotechnology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：24
• 期：4
• 页码：1429-1435
• 全文大小：343 KB
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• 作者单位：Heloísa Bressan Gon?alves (1)
  Jo?o Atílio Jorge (2)
  Luis Henrique Souza Guimar?es (1) (2)
  
  1. Instituto de Química de Araraquara-UNESP, Rua Francisco Degni s/n, 14800-900, Araraquara, S?o Paulo, Brazil
  2. Faculdade de Filosofia, Ciências e Letras de Ribeir?o Preto-USP, Avenida Bandeirantes 3900, Monte Alegre, 14040-901, Ribeir?o Preto, S?o Paulo, Brazil
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

The extracellular β-d-fructofuranosidase from Fusarium graminearum was immobilized using hydrophilic cotton, filter paper, multipurpose cloth, sugar cane bagasse, string, or gauze as alternative cellulosic supports, or with cyanogen bromide agarose. All derivatives (support+enzyme) showed high capacity for reuse (up to 23 times). The derivatives obtained with multipurpose cloth and string were stable at 60°C maintaining 80% of their activity for more than 120 min. The filter paper derivative had a halflife (T50) of 27 min at 70°C. When tested for their pH stability (3.0-.0), all derivatives were more stable than the free enzyme, especially the cotton derivative. The sugarcane bagasse, string, and filter paper derivatives could efficiently produce fructooligosaccharides (FOS) using sucrose as substrate. According to the retention of enzymatic activity, reuse and stabilities, the filter paper and string were the best alternative supports for β-d-fructofuranosidase immobilization, allowing enzyme stabilization and production of FOS.