Fullerene C₆₀ Penetration into Leukemic Cells and Its Photoinduced Cytotoxic Effects

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• 作者：D. Franskevych ; K. Palyvoda ; D. Petukhov ; S. Prylutska…
• 关键词：Fullerene C60 ; Photodynamic therapy ; Leukemic cells ; Protein tyrosine phosphorylation ; Drug resistance ; Mitochondrial membrane potential
• 刊名：Nanoscale Research Letters
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：12
• 期：1
• 全文大小：1388KB
• 刊物主题：Nanotechnology; Nanotechnology and Microengineering; Nanoscale Science and Technology; Nanochemistry; Molecular Medicine;
• 出版者：Springer US
• ISSN：1556-276X
• 卷排序：12

文摘

Fullerene C₆₀ as a representative of carbon nanocompounds is suggested to be promising agent for application in photodynamic therapy due to its unique physicochemical properties. The goal of this study was to estimate the accumulation of fullerene C₆₀ in leukemic cells and to investigate its phototoxic effect on parental and resistant to cisplatin leukemic cells. Stable homogeneous water colloid solution of pristine C₆₀ with average 50-nm diameter of nanoparticles was used in experiments. Fluorescent labeled C₆₀ was synthesized by covalent conjugation of C₆₀ with rhodamine B isothiocyanate. The results of confocal microscopy showed that leukemic Jurkat cells could effectively uptake fullerene C₆₀ from the medium. Light-emitting diode lamp (100 mW cm−2, λ = 420–700 nm) was used for excitation of accumulated C₆₀. A time-dependent decrease of viability was detected when leukemic Jurkat cells were exposed to combined treatment with C₆₀ and visible light. The cytotoxic effect of photoexcited C₆₀ was comparable with that induced by H₂O₂, as both agents caused 50% decrease of cell viability at 24 h at concentrations about 50 μM. Using immunoblot analysis, protein phosphotyrosine levels in cells were estimated. Combined action of C₆₀ and visible light was followed by decrease of cellular proteins phosphorylation on tyrosine residues though less intensive as compared with that induced by H₂O₂ or protein tyrosine kinase inhibitor staurosporine. All tested agents reduced phosphorylation of 55, 70, and 90 kDa proteins while total suppression of 26 kDa protein phosphorylation was specific only for photoexcited C₆₀.