Formaldehyde biodegradation by immobilized Methylobacterium sp. XJLW cells in a three-phase fluidized bed reactor

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• 作者：Lequn Qiu (1)
  Wenwen Chen (1)
  Li Zhong (1)
  Wanxin Wu (1)
  Shijin Wu (1)
  Jianmen Chen (1)
  Fuming Zhang (2)
  Weihong Zhong (1)
  
• 关键词：Methylobacterium sp. XJLW ; Formaldehyde ; Biodegradation ; Immobilized cells ; Three ; phase fluidized bed reactor
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：37
• 期：7
• 页码：1377-1384
• 全文大小：435 KB
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• 作者单位：Lequn Qiu (1)
  Wenwen Chen (1)
  Li Zhong (1)
  Wanxin Wu (1)
  Shijin Wu (1)
  Jianmen Chen (1)
  Fuming Zhang (2)
  Weihong Zhong (1)
  
  1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China
  2. Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA
  
• ISSN：1615-7605

文摘

In the present study, the ability of a newly isolated strain, Methylobacterium sp. XJLW to degrade formaldehyde was investigated in shake flasks and in a bioreactor. The resting cells of Methylobacterium sp. XJLW showed high formaldehyde tolerance (60?g?L?) and high degradation rate (1,687.5?mg?L??h?) in shake flasks. This biodegradation was initiated by a dismutation reaction since formic acid was formed and caused significant dropping of pH in the media. The addition of CaCO3 to the media was found as an effective strategy to control the pH and keep the cells in high degradation bioactivity. A three-phase fluidized bed reactor (TPFBR) was designed to test the formaldehyde-biodegrading ability of immobilized Methylobacterium sp. XJLW. Using a repeated-batch degradation mode, the immobilized cells were able to degrade 5?g?L? formaldehyde (with a maximal degradation rate of 464.5?mg?L??h? under the optimum conditions) and showed stable bioactivity after 20 batches of reuse in the TPFBR.