Acute exposure to silica nanoparticles enhances mortality and increases lung permeability in a mouse model of Pseudomonas aeruginosa pneumonia

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• 作者：Mathilde Delaval (1)
  Sonja Boland (1)
  Brigitte Solhonne (2) (3) (4)
  Marie-Anne Nicola (5)
  St茅phane Mornet (6)
  Armelle Baeza-Squiban (1)
  Jean-Michel Sallenave (2) (3) (4) (7)
  Ignacio Garcia-Verdugo (2) (3) (4) (7)
  
  1. Univ Paris Diderot. Sorbone Paris Cit茅. Unit of Functional and Adaptive Biology (BFA) UMR 8251 ; CNRS ; Laboratory of Molecular and Cellular Responses to Xenobiotics ; 5 rue Thomas Mann ; 75013 ; Paris ; France
  2. Unit茅 de D茅fense Inn茅e et Inflammation ; Institut Pasteur ; 25 rue du Dr Roux ; 75015 ; Paris ; France
  3. INSERM U874 ; Institut Pasteur ; 25 rue du Dr Roux ; 75015 ; Paris ; France
  4. INSERM U1152 ; Facult茅 de M茅dicine site Bichat ; Universit茅 Paris Diderot ; 16 ; rue Henri Huchard ; 75018 ; Paris ; France
  5. Plateforme d鈥檌magerie dynamique ; Institut Pasteur ; 25 rue du Dr Roux ; 75015 ; Paris ; France
  6. CNRS ; Univ. Bordeaux ; ICMCB ; UPR 9048 ; 87 Avenue du Docteur A. Schweitzer ; Pessac cedex ; F-33600 ; France
  7. Universit茅 Sorbonne Paris Cit茅 ; Cellule Pasteur ; Universit茅 Paris Diderot ; rue du Dr Roux ; 75015 ; Paris ; France
  
• 关键词：SiO2 ; Nanoparticles ; Pseudomonas ; Lung ; Inflammation ; Infection ; Alveolar macrophages ; Alveolar permeability
• 刊名：Particle and Fibre Toxicology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：12
• 期：1
• 全文大小：2,826 KB
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• 刊物主题：Pharmacology/Toxicology; Pneumology/Respiratory System; Nanotechnology and Microengineering;
• 出版者：BioMed Central
• ISSN：1743-8977

文摘

Background The lung epithelium constitutes the first barrier against invading pathogens and also a major surface potentially exposed to nanoparticles. In order to ensure and preserve lung epithelial barrier function, the alveolar compartment possesses local defence mechanisms that are able to control bacterial infection. For instance, alveolar macrophages are professional phagocytic cells that engulf bacteria and environmental contaminants (including nanoparticles) and secrete pro-inflammatory cytokines to effectively eliminate the invading bacteria/contaminants. The consequences of nanoparticle exposure in the context of lung infection have not been studied in detail. Previous reports have shown that sequential lung exposure to nanoparticles and bacteria may impair bacterial clearance resulting in increased lung bacterial loads, associated with a reduction in the phagocytic capacity of alveolar macrophages. Results Here we have studied the consequences of SiO2 nanoparticle exposure on Pseudomonas aeruginosa clearance, Pseudomonas aeruginosa-induced inflammation and lung injury in a mouse model of acute pneumonia. We observed that pre-exposure to SiO2 nanoparticles increased mice susceptibility to lethal pneumonia but did not modify lung clearance of a bioluminescent Pseudomonas aeruginosa strain. Furthermore, internalisation of SiO2 nanoparticles by primary alveolar macrophages did not reduce the capacity of the cells to clear Pseudomonas aeruginosa. In our murine model, SiO2 nanoparticle pre-exposure preferentially enhanced Pseudomonas aeruginosa-induced lung permeability (the latter assessed by the measurement of alveolar albumin and IgM concentrations) rather than contributing to Pseudomonas aeruginosa-induced lung inflammation (as measured by leukocyte recruitment and cytokine concentration in the alveolar compartment). Conclusions We show that pre-exposure to SiO2 nanoparticles increases mice susceptibility to lethal pneumonia but independently of macrophage phagocytic function. The deleterious effects of SiO2 nanoparticle exposure during Pseudomonas aeruginosa-induced pneumonia are related to alterations of the alveolar-capillary barrier rather than to modulation of the inflammatory responses.