Keratitis by Fusarium temperatum, a novel opportunist

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• 作者：Abdullah M S Al-Hatmi ; Alexandro Bonifaz ; G Sybren de Hoog…
• 关键词：Keratitis ; Fusarium temperatum ; Maize ; Molecular phylogenetics ; Infection
• 刊名：BMC Infectious Diseases
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,355 KB
• 参考文献：1. Liesegang, TJ, Forster, RF (1980) Spectrum of microbial keratitis in South Florida. Am J Ophthalmol 90: pp. 38-47 CrossRef
  2. Ibrahim, MM, Vanini, R, Ibrahim, FM, Fioriti, LS, Furlan, EM, Provinzano, LM, Castro, RS, Faria, E, Sousa, SJ, Melani Rocha, E (2009) Epidemiologic aspects and clinical outcome of fungal keratitis in southeastern Brazil. Eur J Ophthalmol 19: pp. 355-361
  3. Galarreta, DJ, Tuft, SJ, Ramsay, A, Dart, JK (2007) Fungal keratitis in London: microbiological and clinical evaluation. Cornea 26: pp. 1082-1086 CrossRef
  4. Dóczi, I, Gyetvai, T, Kredics, L, Nagy, E (2004) Involvement of Fusarium spp. in fungal keratitis. Clin Microbiol Infect 10: pp. 773-776 CrossRef
  5. Guarro, J, Gene, J (1995) Opportunistic fusarial infections in humans. Eur J Clin Microbiol Infect Dis 14: pp. 741-754 CrossRef
  6. Bernal, MD, Acharya, NR, Lietman, TM, Strauss, EC, McLeod, SD, Hwang, DG (2006) Outbreak of Fusarium keratitis in soft contact lens wearers in San Francisco. Arch Ophthalmol 124: pp. 1051-1053 CrossRef
  7. Chander, J, Sharma, A (1994) Prevalence of fungal corneal ulcers in northern India. Infection 22: pp. 207-209 CrossRef
  8. Polack, FM, Kaufman, HE, Newmark, E (1971) Keratomycosis. Medical and surgical treatment. Arch Ophthalmol 85: pp. 410-416 CrossRef
  9. Homa, M, Shobana, CS, Singh, YR, Manikandan, P, Selvam, KP, Kredics, L, Narendran, V, Vágv?lgyi, C, Galgóczy, L (2013) Fusarium keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the Fusarium solani species complex. Mycoses 56: pp. 501-11 CrossRef
  10. Kebabc, N, Diepeningen, AD, Ener, B, Ersal, T, Meijer, M, Al-Hatmi, AMS, ?zkocaman, V, Ursava?, A, ?etino?lu, ED, Akal?n, H (2014) Fatal breakthrough infection with Fusarium andiyazi: new multi-resistant aetiological agent cross-reacting with Aspergillus galactomannan enzyme immunoassay. Mycoses 57: pp. 249-255 CrossRef
  11. Jain, PK, Gupta, VK, Misra, AK, Gaur, R, Bajpai, V, Issar, S (2011) Current status of Fusarium infection in human and animal. Asian J Anim Veter Adv 6: pp. 201-227 CrossRef
  12. Nucci, M, Anaissie, E (2007) Fusarium infections in immunocompromised patients. Clin Microbiol Rev 20: pp. 695-704 CrossRef
  13. Mikami, R, Stemmermann, GN (1958) Keratomycosis caused by Fusarium oxysporum. Am J Clin Pathol 29: pp. 257-262
  14. Gupta, AK, Baran, R, Summerbell, RC (2000) Fusarium infections of the skin. Curr Opin Infect Dis 13: pp. 121-128 CrossRef
  15. Nirenberg, HI (1976) Untersuchungen uber die morphologische und biologische differenzierung in der Fusarium Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt für Land- und Forstwirtschaft (Berlin-Dahlem) 169: pp. 1-117
  16. Leslie, JF, Summerell, BA (2006) The Fusarium Laboratory Manual. Blackwell Publishing Ltd, Ames, Oxford CrossRef
  17. O’Donnell, K, Sutton, DA, Rinaldi, MG, Sarver, BA, Balajee, SA, Schroers, HJ, Summerbell, RC, Robert, VA, Crous, PW, Zhang, N, Aoki, T, Jung, K, Park, J, Lee, YH, Kang, S, Park, B, Geiser, DM (2010) Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 48: pp. 3708-3718 CrossRef
  18. Glass, NL, Donaldson, G (1995) Devel
• 刊物主题：Infectious Diseases; Parasitology; Medical Microbiology; Tropical Medicine; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2334

文摘

Background Fusarium species are among the most common fungi present in the environment and some species have emerged as major opportunistic fungal infection in human. However, in immunocompromised hosts they can be virulent pathogens and can cause death. The pathogenesis of this infection relies on three factors: colonization, tissue damage, and immunosuppression. A novel Fusarium species is reported for the first time from keratitis in an agriculture worker who acquired the infection from plant material of maize. Maize plants are the natural host of this fungus where it causes stalk rot and seeding malformation under temperate and humid climatic conditions. The clinical manifestation, microbiological morphology, physiological features and molecular data are described. Methods Diagnosis was established by using polymerase chain reaction of fungal DNA followed by sequencing portions of translation elongation factor 1 alpha (TEF1 α) and beta-tubulin (BT2) genes. Susceptibility profiles of this fungus were evaluated using CLSI broth microdilution method. Results The analyses of these two genes sequences support a novel opportunist with the designation Fusarium temperatum. Phylogenetic analyses showed that the reported clinical isolate was nested within the Fusarium fujikuroi species complex. Antifungal susceptibility testing demonstrated that the fungus had low MICs of micafungin (0.031?μg/ml), posaconazole (0.25?μg/ml) and amphotericin B (0.5?μg/ml). Conclusion The present case extends the significance of the genus Fusarium as agents of keratitis and underscores the utility of molecular verification of these emerging fungi in the human host.