Evaluation of adhesion molecules CD64, CD11b and CD62L in neutrophils and monocytes of peripheral blood for early diagnosis of neonatal infection

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• 作者：Ferah Genel (1) (2)
  Fusun Atlihan (1)
  Nesrin Gulez (1)
  Elif Kazanci (1)
  Canan Vergin (1)
  Demet Tumay Terek (1)
  Ozlem Cengel Yurdun (1)
  
• 关键词：adhesion molecule ; markers ; neonatal infection
• 刊名：World Journal of Pediatrics
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：8
• 期：1
• 页码：72-75
• 全文大小：192KB
• 参考文献：1. Ng PC. Diagnostic markers of infection in neonates. Arch Dis Child Fetal Neonatal Ed 2004;89:F229鈥?35. CrossRef
  2. Fjaertoft G, H氓kansson L, Foucard T, Ewald U, Venge P. CD64 (Fcgamma receptor I) cell surface expression on maturing neutrophils from preterm and term newborn infants. Acta Paediatr 2005;94:295鈥?02. CrossRef
  3. Fjaertoft G, H氓kansson L, Ewald U, Foucard T, Venge P. Neutrophils from term and preterm newborn infants express the high affinity Fcgamma-receptor I (CD64) during bacterial infections. Pediatr Res 1999;45:871鈥?76. CrossRef
  4. Nuutila J, Hohenthal U, Laitinen I, Kotilainen P, Rajam盲ki A, Nikoskelainen J, et al. Simultaneous quantitative analysis of FcgammaRI (CD64) expression on neutrophils and monocytes: a new, improved way to detect infections. J Immunol Methods 2007;328:189鈥?00. CrossRef
  5. Adib M, Ostadi V, Navaei F, Saheb Fosoul F, Oreizi F, Shokouhi R, et al. Evaluation of CD11b expression on peripheral blood neutrophils for early detection of neonatal sepsis. Iran J Allergy Asthma Immunol 2007;6:93鈥?6.
  6. Ng PC, Lam HS. Diagnostic markers for neonatal sepsis. Curr Opin Pediatr 2006;18:125鈥?31. CrossRef
  7. Rodwell RL, Leslie AL, Tudehope DI. Early diagnosis of neonatal sepsis using a hematologic scoring system. J Pediatr 1988;112:761鈥?67. CrossRef
  8. Ng PC, Cheng SH, Chui KM, Fok TF, Wong MY, Wong W, et al. Diagnosis of late onset neonatal sepsis with cytokines, adhesion molecule, and C-reactive protein in preterm very low birthweight infants. Arch Dis Child Fetal Neonatal Ed 1997;77:F221鈥揊227. CrossRef
  9. Spittler A, Razenberger M, Kupper H, Kaul M, Hackl W, Boltz-Nitulescu G, et al. Relationship between interleukin-6 plasma concentration in patients with sepsis, monocyte phenotype, monocyte phagocytic properties, and cytokine production. Clin Infect Dis 2000;31:1338鈥?342. CrossRef
  10. T枚r枚k C, Lundahl J, Hed J, Lagercrantz H. Diversity in regulation of adhesion molecules (Mac-1 and L-selectin) in monocytes and neutrophils from neonates and adults. Arch Dis Child 1993;68:561鈥?65. CrossRef
  11. B眉hrer C, Graulich J, Stibenz D, Dudenhausen JW, Obladen M. L-selectin is down-regulated in umbilical cord blood granulocytes and monocytes of newborn infants with acute bacterial infection. Pediatr Res 1994;36:799鈥?04. CrossRef
  12. Kahn J, Ingraham RH, Shirley F, Migaki GI, Kishimoto TK. Membrane proximal cleavage of L-selectin: identification of the cleavage site and a 6-kD transmembrane peptide fragment of L-selectin. J Cell Biol 1994;125:461鈥?70. CrossRef
  13. Layseca-Espinosa E, P茅rez-Gonz谩lez LF, Torres-Montes A, Baranda L, de la Fuente H, Rosenstein Y, et al. Expression of CD64 as a potential marker of neonatal sepsis. Pediatr Allergy Immunol 2002;13:319鈥?27. CrossRef
  14. Figueras-Aloy J, G贸mez-L贸pez L, Rodr铆guez-Migu茅lez JM, Salvia-Roiges MD, Jord谩n-Garc铆a I, Ferrer-Codina I, et al. Serum soluble ICAM-1, VCAM-1, L-selectin, and P-selectin levels as markers of infection and their relation to clinical severity in neonatal sepsis. Am J Perinatol 2007;24:331鈥?38. CrossRef
  15. Nupponen I, Andersson S, J盲rvenp盲盲 AL, Kautiainen H, Repo H. Neutrophil CD11b expression and circulating interleukin-8 as diagnostic markers for early-onset neonatal sepsis. Pediatrics 2001;108:E12. CrossRef
  16. Ng PC, Li G, Chui KM, Chu WC, Li K, Wong RP, et al. Neutrophil CD64 is a sensitive diagnostic marker for early-onset neonatal infection. Pediatr Res 2004;56:796鈥?03. CrossRef
  17. Turunen R, Andersson S, Nupponen I, Kautiainen H, Siitonen S, Repo H. Increased CD11b-density on circulating phagocytes as an early sign of late-onset sepsis in extremely low-birth-weight infants. Pediatr Res 2005;57:270鈥?75. CrossRef
  18. Weirich E, Rabin RL, Maldonado Y, Benitz W, Modler S, Herzenberg LA, et al. Neutrophil CD11b expression as a diagnostic marker for early-onset neonatal infection. J Pediatr 1998;132:445鈥?51. CrossRef
  19. Ng PC, Li K, Wong RP, Chui KM, Wong E, Fok TF. Neutrophil CD64 expression: a sensitive diagnostic marker for late-onset nosocomial infection in very low birthweight infants. Pediatr Res 2002;51:296鈥?03. CrossRef
  
• 作者单位：Ferah Genel (1) (2)
  Fusun Atlihan (1)
  Nesrin Gulez (1)
  Elif Kazanci (1)
  Canan Vergin (1)
  Demet Tumay Terek (1)
  Ozlem Cengel Yurdun (1)
  
  1. Department of Pediatrics, Dr Beh莽et Uz Children鈥檚 Hospital, Alsancak, Izmir, Turkey
  2. Department of Pediatrics, Dr Beh莽et Uz Children鈥檚 Hospital, 1374 sokak, No: 11, 35210, Alsancak, Izmir, Turkey
  

文摘

Background This study was undertaken to assess the value of neutrophils CD11b, CD64, and CD62L for the early diagnosis of neonatal infection. Methods Eighty-four neonates who were followed up for a suspected neonatal infection were included in this study. They were assigned into an infection group (n=49) and a non-infection group (n=35). Healthy neonates served as controls (n=35). A full sepsis screening was performed and neutrophil and monocyte expressions of CD11b, CD64 and CD62L were determined by flow cytometry. Results The expressions of CD64 and CD11b were significantly enhanced in the infection group compared to the non-infective group and the controls. Conclusions CD64 expression on neutrophils and monocytes is a useful diagnostic marker for the early diagnosis of neonatal infection. Combination of CD64, CD11b and C reactive protein further enhances the sensitivity of the expression and its negative predictive value.