Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.)

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• 作者：Yanchun You (1) (2) (3)
  Miao Xie (1) (2) (3)
  Nana Ren (1) (3)
  Xuemin Cheng (1) (3)
  Jianyu Li (1) (2) (3)
  Xiaoli Ma (1) (3)
  Minming Zou (1) (3)
  Liette Vasseur (1) (4)
  Geoff M Gurr (1) (3) (5)
  Minsheng You (1) (3)
  
  1. Institute of Applied Ecology and Research Centre for Biodiversity and Eco-Safety ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
  2. College of Life Science ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
  3. Key Laboratory of Integrated Pest Management of Fujian and Taiwan ; China Ministry of Agriculture ; Fuzhou ; 350002 ; China
  4. Department of Biological Sciences ; Brock University ; 500 Glenridge Avenue ; St. Catharines ; ON ; L2S 3A1 ; Canada
  5. EH Graham Centre ; Charles Sturt University ; Orange ; NSW ; 2800 ; Australia
  
• 关键词：Transcriptome analysis ; qRT ; PCR ; Phylogenetic analysis ; Insect pest ; Lepidoptera
• 刊名：BMC Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：16
• 期：1
• 全文大小：3,070 KB
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• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Glutathione S-transferases (GSTs) are multifunctional detoxification enzymes that play important roles in insects. The completion of several insect genome projects has enabled the identification and characterization of GST genes over recent years. This study presents a genome-wide investigation of the diamondback moth (DBM), Plutella xylostella, a species in which the GSTs are of special importance because this pest is highly resistant to many insecticides. Results A total of 22 putative cytosolic GSTs were identified from a published P. xylostella genome and grouped into 6 subclasses (with two unclassified). Delta, Epsilon and Omega GSTs were numerically superior with 5 genes for each of the subclasses. The resulting phylogenetic tree showed that the P. xylostella GSTs were all clustered into Lepidoptera-specific branches. Intron sites and phases as well as GSH binding sites were strongly conserved within each of the subclasses in the GSTs of P. xylostella. Transcriptome-, RNA-seq- and qRT-PCR-based analyses showed that the GST genes were developmental stage- and strain-specifically expressed. Most of the highly expressed genes in insecticide resistant strains were also predominantly expressed in the Malpighian tubules, midgut or epidermis. Conclusions To date, this is the most comprehensive study on genome-wide identification, characterization and expression profiling of the GST family in P. xylostella. The diversified features and expression patterns of the GSTs are inferred to be associated with the capacity of this species to develop resistance to a wide range of pesticides and biological toxins. Our findings provide a base for functional research on specific GST genes, a better understanding of the evolution of insecticide resistance, and strategies for more sustainable management of the pest.