Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin
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- 作者：Amélie Carbonnelle ; Nadine Martin-Garcia ; Nicolas Ortonne ; Liliane Laroche ; Martine Bagot ; Valérie Molinier-Frenkel and Janine Wechsler
- 关键词：Dermathopathology ; Lymphoma ; Morphometry
- 刊名：Virchows Archiv
- 出版年：2007
- 出版时间：April, 2007
- 年：2007
- 卷：450
- 期：4
- 页码：441-447
- 全文大小：437 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Pathology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-2307
文摘
Distinguishing between low-grade primary cutaneous B-cell lymphoma (LG-pCBCL) and cutaneous lymphoid hyperplasia (CLH) based on histological features is often difficult. CLH lesions contain numerous reactive cells of the histiocyte lineage [Langerhans cells (LC), dermal dendritic cells (DDC), and macrophages], which are also often present in CBCL. The aim of this study was to determine whether immunohistochemical detection of those cells could help differentiate between CLH and LG-pCBCL. We determined the percentages of those histiocytic cells in the dermal infiltrates of 45 cases of cutaneous lymphoproliferations comprising 16 CLH and 29 LG-pCBCL (19 follicle-center cell lymphomas and 10 marginal zone lymphomas) by immunohistochemical labeling with antibodies to CD1a, FXIIIa, and CD68 to respectively detect LC, DDC, and macrophages. To avoid observer-dependent bias, an automated morphometric analysis method was used to recognize immunoreactive cells and calculate their percentages within the infiltrate. FXIIIa+ cells were significatively more frequent in CLH than in LG-pCBCL, whereas CD1a+ and CD68+ cell frequencies were comparable in the two groups. The results of our study suggest that DDC might play an important role in the genesis of cutaneous lymphomas.