Polyethylene glycols interact with membrane glycerophospholipids: is this part of their mechanism for hypothermic graft protection?

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• 作者：Delphine Dutheil (1) (2) (5)
  Anja Underhaug Gjerde (4)
  Isabelle Petit-Paris (1) (3)
  Gérard Mauco (1) (2) (3)
  Holm Holmsen (4)
  
• 关键词：Organ transplantation ; Polyethylene Glycol ; Hypothermia ; Membrane Fluidity ; Glycerophospholipid
• 刊名：Journal of Chemical Biology
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：2
• 期：1
• 页码：39-49
• 全文大小：499KB
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• 作者单位：Delphine Dutheil (1) (2) (5)
  Anja Underhaug Gjerde (4)
  Isabelle Petit-Paris (1) (3)
  Gérard Mauco (1) (2) (3)
  Holm Holmsen (4)
  
  1. Inserm U927, Poitiers, France
  2. Université de Poitiers, Faculté de Médecine et de Pharmacie, Poitiers, France
  5. Hemarina SA, Morlaix, France
  4. Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009, Bergen, Norway
  3. CHU Poitiers, Laboratoire de Biochimie et Toxicologie, Poitiers, France
  
• ISSN：1864-6166

文摘

Polyethylene glycol (PEG), a high-molecular-weight colloid present in new organ preservation solutions, protects against cold ischemia injuries leading to better graft function of transplanted organs. This protective effect cannot be totally explained by immuno-camouflaging property or signaling-pathway modifications. Therefore, we sought for an alternative mechanism dependent on membrane fluidity. Using the Langmuir–Pockles technique, we show here that PEGs interacted with lipid monolayers of defined composition or constituted by a renal cell lipid extract. High-molecular-weight PEGs stabilized the lipid monolayer at low surface pressure. Paradoxically, at high surface pressure, PEGs destabilized the monolayers. Hypothermia reduced the destabilization of saturated monolayer whereas unsaturated monolayer remained unaffected. Modification of ionic strength and pH induced a stronger stabilizing effect of PEG 35,000?Da which could explain its reported higher effectiveness on cold-induced injuries during organ transplantation. This study sheds a new light on PEG protective effects during organ preservation different from all classical hypotheses.