Tau is Endogenously Nitrated in Mouse Brain: Identification of a Tyrosine Residue Modified In?vivo by NO

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• 作者：Simona Nonnis ; Graziella Cappelletti ; Francesca Taverna ; Cristina Ronchi ; Severino Ronchi ; Armando Negri ; Eleonora Grassi and Gabriella Tedeschi
• 关键词：Tau ; Nitric oxide ; Brain ; Cytoskeleton ; MALDI ; TOF ; PC12 cells
• 刊名：Neurochemical Research
• 出版年：2008
• 出版时间：March, 2008
• 年：2008
• 卷：33
• 期：3
• 页码：518-525
• 全文大小：278.9 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Biochemistry
  Neurology
  
• 出版者：Springer Netherlands
• ISSN：1573-6903

文摘

Nitration of tau protein is normally linked to neurodegeneration but, until now, no comprehensive information is available regarding tau nitration in healthy subjects. It has been previously reported that in differentiated PC12 cells, tau co-immunoprecipitated with alpha-tubulin is nitrated at tyrosine residues and that this post-translation modification doesn’t impair the association of tau with the cytoskeleton. The present paper is focused on the identification of tyrosine residues endogenously modified in tau from PC12 cells and reports for the first time that tau is also nitrated in vivo in normal mouse brain and that one tyrosine is endogenously modified.