Galleria mellonella Larvae as an Infection Model for Penicillium marneffei

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• 作者：Xiaowen Huang ; Dedong Li ; Liyan Xi ; Eleftherios Mylonakis
• 关键词：Penicillium marneffei ; Galleria mellonella ; Infection model
• 刊名：Mycopathologia
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：180
• 期：3-4
• 页码：159-164
• 全文大小：3,101 KB
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  24.Huang
• 作者单位：Xiaowen Huang (1) (2)
  Dedong Li (2) (3)
  Liyan Xi (1)
  Eleftherios Mylonakis (2)
  
  1. Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 West Yanjiang Road, Guangzhou, China
  2. Division of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School of Brown University, 593 Eddy Street, Providence, RI, 02903, USA
  3. Department of Clinical Pharmacology, PLA General Hospital, 28 Fu Xing Road, Beijing, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Penicillium marneffei, the only known dimorphic and pathogenic species in the genus of Penicillium, is responsible for severe to deadly infection in immunocompromised patients. In this study, P. marneffei was able to infect the greater wax moth Galleria mellonella. The increasing inoculum doses of P. marneffei cells resulted in greater larval mortality, and the larval mortality rate also depended on the incubation temperature after P. marneffei infection and different P. marneffei strains. Moreover, the phagocytosis of hemocytes to P. marneffei was investigated, and it showed that the phagocytosis was increasing during the infection. These results demonstrated that G. mellonella can be effectively used to facilitate the in vivo study of P. marneffei infection and hemocytes are the key component of the larvae’s immune defenses. Keywords Penicillium marneffei Galleria mellonella Infection model