Suitability of enzymatic markers to assess the environmental condition of natural populations of Gambusia affinis and Daphnia magna—a case study

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• 作者：Inês Domingues ; Cátia S. A. Santos…
• 关键词：Environmental parameters ; Chemical stress ; Biochemical markers ; Daphnia magna ; Gambusia affinis
• 刊名：Environmental Monitoring and Assessment
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：187
• 期：4
• 全文大小：1,516 KB
• 参考文献：1. Adams, S. M. (2000). Assessing sources of stress to aquatic ecosystems using integrated biomarkers. / Biological Resource Management: Connecting Science and Policy 17-9.
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  16. Diamantino
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Atmospheric Protection, Air Quality Control and Air Pollution
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-2959

文摘

In recent years, the use of biochemical markers, especially in the assessment of toxic effects and modes of action, under controlled laboratory conditions has increased. However, transposing their use to in situ monitoring or risk assessment evaluations has encountered barriers, mainly related to the difficulty in interpreting the meaning of biochemical variation. In this work, we aimed at understanding if biochemical marker activities (cholinesterase, glutathione S-transferase and lactate dehydrogenase) can be used to monitor the health status of natural populations of fish (Gambusia affinis) and daphnids (Daphnia magna). For that, two ponds with different water properties were chosen as study sites, and organisms collected at four sampling periods along the year. The pattern of biochemical marker responses was not the same in the two species, showing higher integrated biochemical marker response values in the winter for G. affinis and in the autumn for D. magna, suggesting specificities that must be taken into account in biomonitoring programmes by including representative species of several trophic levels. In the case of G. affinis, the differences in key physicochemical parameters between the two ponds (especially dissolved oxygen levels) did not seem to affect biochemical marker levels as if organisms were already perfectly adapted to their environment. In general, seasonal variation of water quality seems to have an important role on biochemical marker responses. Several parameters above Environmental Quality Standards were identified such as dissolved oxygen (DO), ammonia, nitrites, sulphides and metals, but eventual responses to these stressors could not be discriminated from natural variation except for particular cases.