Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri

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• 作者：Gaowa Saren (1) (2)
  Yonggang Zhao (3)
  
• 关键词：amphioxus B. belcheri ; Amphi ; PhLP ; northern blotting ; in situ hybridization ; cellular localization
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2009
• 出版时间：May 2009
• 年：2009
• 卷：27
• 期：2
• 页码：324-330
• 全文大小：1070KB
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• 作者单位：Gaowa Saren (1) (2)
  Yonggang Zhao (3)
  
  1. Department of Marine Biology, Ocean University of China, Qingdao, 266003, China
  2. Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  3. China Animal Health and Epidemiology Center, Qingdao, 266032, China
  
• ISSN：1993-5005

文摘

A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.