Requirement of dual stimulation by homologous recombinant IL-2 and recombinant IL-12 for the in vitro production of interferon gamma by canine peripheral blood mononuclear cells

详细信息    查看全文

• 作者：Andrea Mendes Pereira ; Cristiane Garboggini Melo de Pinheiro…
• 关键词：Interleukin 2 ; Interleukin 12 ; Cellular immune response ; Dog
• 刊名：BMC Research Notes
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：767 KB
• 参考文献：1. Dinarello, CA (2007) Historical insights into cytokines. Eur J Immunol 37: pp. S34-S45 CrossRef
  2. Boyman, O, Purton, JF, Surh, CD, Sprent, J (2007) Cytokines and T-cell homeostasis. Curr Opin Immunol 19: pp. 320-326 CrossRef
  3. Bezbradica, JS, Medzhitov, R (2009) Integration of cytokine and heterologous receptor signaling pathways. Nat Immunol 10: pp. 333-339 CrossRef
  4. Paul, WE (2008) Fundamental Immunoloy. Lippincott Williams & Wilkins, Philadelphia
  5. Watson, J, Gillis, S, Marbrook, J, Mochizuki, D, Smith, KA (1979) Biochemical and biological characterization of lymphocyte regulatory molecules. I. purification of a class of murine lymphokines. J Exp Med 150: pp. 849-861 CrossRef
  6. Taniguchi, T, Matsui, H, Fujita, T, Takaoka, C, Kashima, N, Yoshimoto, R, Hamuro, J (1983) Structure and expression of a cloned cDNA for human interleukin-2. Nature 302: pp. 305-310 CrossRef
  7. Sojka, DK, Bruniquel, D, Schwartz, RH, Singh, NJ (2004) IL-2 secretion by CD4+ T cells in vivo is rapid, transient, and influenced by TCR-specific competition. J Immunol 172: pp. 6136-6143 CrossRef
  8. D’Souza, WN, Lefrancois, L (2004) Frontline: an in-depth evaluation of the production of IL-2 by antigen-specific CD8 T cells in vivo. Eur J Immunol 34: pp. 2977-2985 CrossRef
  9. Boyman, O, Sprent, J (2012) The role of interleukin-2 during homeostasis and activation of the immune system. Nat Rev Immunol 12: pp. 180-190
  10. Trinchieri, G, Gerosa, F (1996) Immunoregulation by interleukin-12. J Leukoc Biol 59: pp. 505-511
  11. Trinchieri, G (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 3: pp. 133-146 CrossRef
  12. Helfand, SC, Modiano, JF, Nowell, PC (1992) Immunophysiological studies of interleukin-2 and canine lymphocytes. Vet Immunol Immunopathol 33: pp. 1-16 CrossRef
  13. Helfand, SC, Soergel, SA, Modiano, JF, Hank, JA, Sondel, PM (1994) Induction of lymphokine-activated killer (LAK) activity in canine lymphocytes with low dose human recombinant interleukin-2 in vitro. Cancer Biother 9: pp. 237-244 CrossRef
  14. G. R. Cain, TK, N Taylor, I, Champlin, R (1992) Effects of administration of Recombinant Human Interleukin-2 in dogs. Comp Haematol Int 2: pp. 201-207 CrossRef
  15. Ohnishi, H, Okuno, K, Yasutomi, M (1993) Successful in vivo generation of canine lymphokine-activated killer cells by continuous recombinant interleukin-2 infusion through the splenic artery. Cancer Biother 8: pp. 213-222 CrossRef
  16. Helfand, SC, Soergel, SA, MacWilliams, PS, Hank, JA, Sondel, PM (1994) Clinical and immunological effects of human recombinant interleukin-2 given by repetitive weekly infusion to normal dogs. Cancer Immunol Immunother 39: pp. 84-92 CrossRef
  17. Phillips, BS, Padilla, ML, Dickerson, EB, Lindstrom, MJ, Helfand, SC (1999) Immunostimulatory effects of human recombinant interleukin-12 on peripheral blood mononuclear cells from normal dogs. Vet Immunol Immunopathol 70: pp. 189-201 CrossRef
  18. Cemazar, MSG, Pavlin, D, Tozon, N Intramuscular IL-12 Electrogene Therapy for Treatment of Spontaneous Canine Tumors. In: You, Y eds. (2011) Targets in gene therapy. Croatia: Rijeka: InTech, Rijeka, pp. 299-320
  19. Okano, F, Satoh, M, Yamada, K (1997) Cloning and ex
• 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500

文摘

Background Very few studies have been carried out so far aiming at modulating cellular immune responses in dogs. In this study, we evaluated the ability of recombinant canine IL-2 (rcaIL-2) and IL-12, in the form of a single-chain fusion protein (rsccaIL-12), to stimulate peripheral blood mononuclear cells (PBMC) of healthy mongrel dogs. Results Recombinant canine IL-2 purified from Escherichia coli or present in the supernatant of COS-7 cells transfected with pcDNA3.1-caIL-2 (COS-7 caIL-2 supernatant) was able to induce proliferation of CTLL-2 cells, thus showing their functional activity. In addition, purified rcaIL-2 and COS-7 caIL-2 supernatant stimulated resting canine PBMC proliferation to a level higher than baseline level. Neither COS-7 sccaIL-12 supernatant nor COS-7 caIL-2 supernatant alone was able to induce significant production of interferon gamma by resting PBMC. However, COS-7 sccaIL-12 supernatant in combination with COS-7 caIL-2 supernatant induced production of IFN-γ by those cells. Conclusions The data shown herein suggest that the combination of canine recombinant IL-12 and IL-2 can be useful to promote cellular immune responses in dogs.