Targeted amplification for enhanced detection of biothreat agents by next-generation sequencing
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- 作者：Shea N. Gardner ; Kenneth G. Frey ; Cassie L. Redden ; James B. Thissen…
- 关键词：Targeted amplification ; Biodefense ; Next Generation Sequencing
- 刊名：BMC Research Notes
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：8
- 期：1
- 全文大小：1,315 KB
- 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1756-0500
文摘
Background Historically, identification of causal agents of disease has relied heavily on the ability to culture the organism in the laboratory and/or the use of pathogen-specific antibodies or sequence-based probes. However, these methods can be limiting: Even highly sensitive PCR-based assays must be continually updated due to signature degradation as new target strains and near neighbors are sequenced. Thus, there has been a need for assays that do not suffer as greatly from these limitations and/or biases. Recent advances in library preparation technologies for Next-Generation Sequencing (NGS) are focusing on the use of targeted amplification and targeted enrichment/capture to ensure that the most highly discriminating regions of the genomes of known targets (organism-unique regions and/or regions containing functionally important genes or phylogenetically-discriminating SNPs) will be sequenced, regardless of the complex sample background.