Characterization of microsatellite markers for Saxicola species

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• 作者：Kim G. Mortega ; Gavin J. Horsburgh ; Juan C. Illera…
• 关键词：Microsatellite ; Passerine ; Predicted genome locations ; Saxicola torquata ; Sequence tandem repeats (STR) ; Songbird
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：273-278
• 全文大小：181 KB
• 参考文献：1. Dawson DA (2007) Genomic analysis of passerine birds using conserved microsatellite loci. PhD Thesis, University of Sheffield, UK
  2. Dawson DA, Burke T, Hansson B, Pandhal J, Hale MC, Hinten GN, Slate J (2006) A predicted microsatellite map of the passerine genome based on chicken–passerine sequence similarity. Mol Ecol 15:1299-320 ss="external" href="http://dx.doi.org/10.1111/j.1365-294X.2006.02803.x" target="_blank" title="It opens in new window">CrossRef
  3. Illera JC, Richardson DS, Helm B, Atienza JC, Emersona BC (2008) Phylogenetic relationships, biogeography and speciation in the avian genus / Saxicola. Mol Phylogenet Evol 48:1145-154 ss="external" href="http://dx.doi.org/10.1016/j.ympev.2008.05.016" target="_blank" title="It opens in new window">CrossRef
  4. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365-86
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

We characterized 28 unique African stonechat (Saxicola torquata) microsatellite loci. Seventeen loci characterized in 24 unrelated Saxicola torquata axillaris individuals sampled at Mount Meru, Tanzania displayed 2-6 alleles per locus and observed heterozygosities ranged from 0.29 to 0.92. Heterozygous females and sequence similarity suggested all 17 loci were autosomal. All markers also successfully amplified in nine different species ranging from Europe to Asia, including three endemic Island species. These microsatellite markers will be useful to assess the genetic diversity of the large and widely distributed genus Saxicola, a group comprising 11 recognized species with evidences of cryptic diversification. Several species show a narrow range distribution and are of conservation concern.