Autosomal and Z-linked microsatellite markers enhanced for cross-species utility and assessed in a range of birds, including species of conservation concern

详细信息    查看全文

• 作者：Deborah A. Dawson ; Susannah Bird ; Gavin J. Horsburgh…
• 关键词：AVES ; Birds ; High cross ; species utility ; Passerine ; Simple tandem repeat (STR) ; Z chromosome
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：7
• 期：4
• 页码：881-886
• 全文大小：343 KB
• 参考文献：Backstr?m N, Brandstr?m M, Gustafsson L, Qvarnstr?m A, Cheng H, Ellegren H (2006) Genetic mapping in a natural population of collared flycatchers (Ficedula albicollis): conserved synteny but gene order rearrangements on the avian Z chromosome. Genetics 174:377-86PubMedCentral CrossRef PubMed
  Ball AD, Stapley J, Dawson DA, Birkhead TR, Burke T, Slate J (2010) A comparison of SNPs and microsatellites as linkage mapping markers: lessons from the zebra finch (Taeniopygia guttata). BMC Genom 11:218CrossRef
  Burgess SL, Fleischer RC (2006) Isolation and characterization of polymorphic microsatellite loci in the Hawaiian flycatcher, the elepaio (Chasiempis sandwichensis). Mol Ecol Notes 6:14-6CrossRef
  Dawson DA (2007) Genomic analysis of passerine birds using conserved microsatellite loci. PhD Thesis, University of Sheffield, UK
  Dawson DA, Horsburgh GJ, Küpper C, Stewart IRK, Ball AD, Durrant KL, Hansson B, Bacon I, Bird S, Klein á, Lee J-W, Martín-Gálvez D, Simeoni M, Smith G, Spurgin LG, Burke T (2010) New methods to identify conserved microsatellite loci and develop primer sets of high utility—as demonstrated for birds. Mol Ecol Resour 10:475-94CrossRef PubMed
  IUCN (International Union for Conservation of Nature and Natural Resources) (2014) The IUCN red list of threatened species. Version 2014.2 (http://?www.?iucnredlist.?org/?search ) Accessed 9th Oct 2014
  King TL, Eackles MS, Henderson AP, Bocetti CI, Currie D, Wunderle JM (2005) Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland’s warbler (Dendroica kirtlandii). Mol Ecol Notes 5:569-71CrossRef
  Lifjeld JT, Marthinsen G, Myklebust M, Dawson DA, Johnsen A (2010) A wild Marsh Warbler × Sedge Warbler hybrid (Acrocephalus palustris × A. schoenobaenus) in Norway documented with molecular markers. J Ornithol 151:513-17CrossRef
  Otsuka R, Nishiumi I, Wada M (2003) Characterization of 12 polymorphic microsatellite loci in the Japanese bush warbler Cettia diphone. Mol Ecol Notes 3:44-6CrossRef
  Replogle K, Arnold AP, Ball GF et al (2008) The Songbird Neurogenomics (SoNG) Initiative: community-based tools and strategies for study of brain gene function and evolution. BMC Genom 9:131CrossRef
  Richardson DS, Jury FL, Dawson DA, Salguiero P, Komdeur J, Burke T (2000) Fifty Seychelles warbler (Acrocephalus sechellensis) microsatellite loci polymorphic in Sylviidae species and their cross-species amplification in other passerine birds. Mol Ecol 9:2226-231CrossRef PubMed
  Stenzler LM, Fitzpatrick JW (2002) Isolation of microsatellite loci in the Florida Scrub-Jay Aphelocoma coerulescens. Mol Ecol Notes 2:547-50CrossRef
  
• 作者单位：Deborah A. Dawson (1)
  Susannah Bird (1) (2)
  Gavin J. Horsburgh (1)
  Alexander D. Ball (1)
  
  1. Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
  2. Department of Biology, University of York, York, YO10 5DD, UK
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Microsatellite markers were designed to be of utility for genotyping multiple species of birds, including those of conservation concern, hence saving resources and enabling species/genome comparisons. We used the proven approach of Dawson et al. (Mol Ecol Resour 10:475-94, 2010) and assessed markers in multiple species, including nine species of conservation interest. We ensured both primer sequences matched multiple species (13 loci) or designed primer sets from expressed sequence tags (2 loci). Eleven primer sets were 100 % identical to the zebra finch (Taeniopygia guttata) and a second passerine species and/or the chicken (Gallus gallus). All 15 loci were polymorphic when assessed in a non-source species (Gouldian finch, Erythrura gouldiae) suggesting utility in multiple species. Four of the five Z-linked loci were assessed in at least nine additional species each (including ratites). All were variable in multiple species, demonstrating cross-species utility and potential for identifying Z chromosome rearrangements. Keywords AVES Birds High cross-species utility Passerine Simple tandem repeat (STR) Z chromosome