A panel of new microsatellite loci for genetic studies of antarctic fur seals and other otariids

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• 作者：Joseph I. Hoffman
• 关键词：Arctocephalus gazella ; Microsatellite ; Cross ; species amplification ; Stock structure ; Assignment testing ; Heterozygosity ; fitness correlation (HFC) ; Pinniped
• 刊名：Conservation Genetics
• 出版年：2009
• 出版时间：August, 2009
• 年：2009
• 卷：10
• 期：4
• 页码：989-992
• 全文大小：150.6 KB

文摘

Nine dinucelotide microsatellite loci were developed in the Antarctic fur seal Arctocephalus gazella. Each locus possessed between 4 and 9 alleles in a sample of twenty individuals sampled from Bird Island, South Georgia, and expected heterozygosity ranged from 0.52 to 0.84. All but one of the loci conformed to Hardy–Weinberg equilibrium and no evidence was found for genotypic disequilibrium. Additionally, all of the loci successfully cross-amplified in the South American fur seal Arctocephalus australis, the New Zealand fur seal Arctocephalus forsteri and the Steller’s sea lion Eumetopias jubatus, and six also yielded products in the more distantly related harbour seal Phoca vitulina and walrus Odobenus rosmarus. These loci should prove useful for studies of the population genetics of Antarctic fur seals and other important otariid species.