Chemical imaging of lipid droplets in muscle tissues using hyperspectral coherent Raman microscopy

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• 作者：Nils Billecke (1)
  Gianluca Rago (1) (2) (3)
  Madeleen Bosma (4)
  Gert Eijkel (2)
  Anne Gemmink (5)
  Philippe Leproux (6) (7)
  Guillaume Huss (6)
  Patrick Schrauwen (4)
  Matthijs K. C. Hesselink (5)
  Mischa Bonn (1)
  Sapun H. Parekh (1)
  
• 关键词：Lipid droplet ; Microscopy ; Chemical imaging ; Raman spectroscopy ; Multivariate analysis ; Hyperspectral
• 刊名：Histochemistry and Cell Biology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：141
• 期：3
• 页码：263-273
• 全文大小：866 KB
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• 作者单位：Nils Billecke (1)
  Gianluca Rago (1) (2) (3)
  Madeleen Bosma (4)
  Gert Eijkel (2)
  Anne Gemmink (5)
  Philippe Leproux (6) (7)
  Guillaume Huss (6)
  Patrick Schrauwen (4)
  Matthijs K. C. Hesselink (5)
  Mischa Bonn (1)
  Sapun H. Parekh (1)
  
  1. Molecular Spectroscopy Department, Max Plank Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany
  2. FOM Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, The Netherlands
  3. Deloitte Consulting B.V., Laan van Kronenburg 2, 1183 AS, Amstelveen, The Netherlands
  4. Department of Human Biology, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center, 6200 MD, Maastricht, The Netherlands
  5. Department of Movement Sciences, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center, 6200 MD, Maastricht, The Netherlands
  6. Leukos Innovative Optical Systems, ESTER Technopole, 1 avenue d鈥橢ster, 87069, Limoges Cedex, France
  7. Xlim Research Institute, CNRS-University of Limoges, 123 Avenue Albert Thomas, 87060, Limoges Cedex, France
  
• ISSN：1432-119X

文摘

The accumulation of lipids in non-adipose tissues is attracting increasing attention due to its correlation with obesity. In muscle tissue, ectopic deposition of specific lipids is further correlated with pathogenic development of insulin resistance and type 2 diabetes. Most intramyocellular lipids are organized into lipid droplets (LDs), which are metabolically active organelles. In order to better understand the putative role of LDs in pathogenesis, insight into both the location of LDs and nearby chemistry of muscle tissue is very useful. Here, we demonstrate the use of label-free coherent anti-Stokes Raman scattering (CARS) microscopy in combination with multivariate, chemometric analysis to visualize intracellular lipid accumulations in ex vivo muscle tissue. Consistent with our previous results, hyperspectral CARS microscopy showed an increase in LDs in tissues where LD proteins were overexpressed, and further chemometric analysis showed additional features morphologically (and chemically) similar to mitochondria that colocalized with LDs. CARS imaging is shown to be a very useful method for label-free stratification of ectopic fat deposition and cellular organelles in fresh tissue sections with virtually no sample preparation.