Cyclobacterium sediminis sp. nov. isolated from a sea cucumber aquaculture farm and emended description of the genus Cyclobacterium

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• 作者：Seyeon Shin ; Hyung-Yeel Kahng
• 关键词：polyphasic study ; sea cucumber ; aquaculture farm sediment ; Cyclobacterium sediminis sp. nov.
• 刊名：Journal of Microbiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：55
• 期：2
• 页码：90-95
• 全文大小：
• 刊物主题：Microbiology;
• 出版者：The Microbiological Society of Korea
• ISSN：1976-3794
• 卷排序：55

文摘

An aerobic, Gram-negative bacterium, designated strain SD70T, was isolated from sea cucumber aquaculture farm sediment in Taean, Korea, and its taxonomic status was established by undertaking a polyphasic study. Cells of strain SD70T were non-motile, catalase-, and oxidase-positive, non-spore-forming, and horseshoe-shaped. Optimal growth was observed under 25–30°C, pH 7.0–8.0, and 3.0–5.0% (w/v) NaCl conditions. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SD70T fell within an evolutionary group comprising species of the genus Cyclobacterium. Strain SD70T shared 92.1–98.5% 16S rRNA sequence similarity values with the type strains of species of the genus Cyclobacterium. Relatively low levels of DNA-DNA relatedness were found between strain SD70T and C. marinum DSM 745T (40.2%) and C. amurskyense KMM 6143T (15.8%). The predominant cellular fatty acids were iso-C_15:0 (32.1%), and anteiso-C_15:0 (9.1%). Menaquinone MK-7 was the only respiratory quinone. The G+C content of the genomic DNA was 36 mol%. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and seven unidentified lipids. On the basis of phenotypic and genotypic data, strain SD70T represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium sediminis sp. nov. is proposed. An emended description of the genus Cyclobacterium is also provided.